橙黄乳菇对马尾松和华山松幼苗生长及根系代谢物的调控

doi:10.11707/j.1001-7488.LYKX20230385

Abstract

Abstract:

Objective: The study is aimed to investigate the effects of Lactarius akahatsu inoculation on the growth, root development, and metabolites of Pinus massoniana and Pinus armandii seedlings, and to provide a scientific basis to cultivate pine mycorrhizal seedlings and symbiotic cultivation of Lactarius akahatsu. Method: Potted seedlings of Pinus massoniana and Pinus armandii were placed in the greenhouse and applied with two treatments of Lactarius akahatsu inoculation and non-inoculation, the difference in the mycorrhizal colonization rate and mycorrhizal dependency, shoot and root growth indicators, the root architecture traits (total root length, root surface area, root volume, root tips, bifurcation), the root morphology traits (specific root length, root tissue density, specific root surface area) and main root metabolites were studied. Result: Evident mycorrhizal morphology of the Lactarius akahatsu was formed in the seedlings of the two pines, and displayed a high mycorrhizal colonization rate and mycorrhizal dependency. The inoculation of Lactarius akahatsu promoted the growth of seedlings to a certain extent, but significant differences were found between the two species. Lactarius akahatsu inoculation significantly increased the plant seedling’s height and dry aboveground weight of Pinus massoniana seedlings, whereas the plant height and dry underground weight of Pinus armandii. The total root length, root surface area, root volume, root tip number, and bifurcation of the two pine species increased by Lactarius akahatsu inoculation, while root vigor and cation exchange capacity decreased. Inoculation with Lactarius akahatsu significantly affected the content of main metabolites in the roots of the two pine species. After Lactarius akahatsu inoculation, the total phenols content and total flavonoid content in the roots of Pinus massoniana seedlings decreased, while the total triterpenoid content, total alkaloid content and total sterol content increased. The trends in metabolites in the root of Pinus armandii seedlings was opposite to that of Pinus massoniana seedlings. Conclusion: Lactarius akahatsu inoculation improves the root morphology and biomass of the seedlings, and affects the content of main metabolites in the roots, and therefore Lactarius akahatsu inoculation is feasible to cultivate pine mycorrhizal seedlings and symbiotic cultivation of ectomycorrhizal fungi.

Key words: Lactarius akahatsu, ectomycorrhizal fungi, metabolites, growth regulation, Pinus massoniana, Pinus armandii

CLC Number:

S718.81

Shixin Zhang,Yangyang Geng,Ting Zhou,Jihui Wang,Bokai Hu,Yana Liu. Regulation of Lactarius akahatsu on the Growth and Root Metabolites of Pinus massoniana and Pinus armandii Seedlings[J]. Scientia Silvae Sinicae, 2024, 60(9): 50-58.

Figures/Tables 9

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处理 Treatment	株高 Plant height/cm	地径 Ground diameter/ cm	地上部分Shoot		地下部分Root		总生物量 Total biomass/g
处理 Treatment	株高 Plant height/cm	地径 Ground diameter/ cm	鲜质量 Fresh weight/g	干质量 Dry weight/g	鲜质量 Fresh weight/g	干质量 Dry weight/g	总生物量 Total biomass/g
PM	7.19±1.56b	1.13±0.24a	0.31±0.13b	0.13±0.06b	0.77±0.32a	0.13±0.05a	0.26±0.10a
PM×LA	9.59±1.69a	1.15±0.18a	0.45±0.15a	0.17±0.06a	0.71±0.34a	0.14±0.07a	0.31±0.12a
PA	10.50±2.03a	2.07±0.23a	1.36±0.36a	0.52±0.16a	2.31±0.95b	0.47±0.17b	0.99±0.17a
PA×LA	11.01±1.90a	2.09±0.23a	1.44±0.94a	0.59±0.14a	2.86±0.78a	0.63±0.15a	1.15±0.15a

处理 Treatment	总根长 Total root length/cm	根表面积 Root surface area/cm²	平均直径 Average diameter/mm	体积 Volume/mm³	根尖数 Tips	分叉数 Bifurcation	根部含水率 Root moisture content(%)
PM	18.91±2.01b	24.10±4.89b	0.44±0.05a	0.29±0.02b	909.44±15.91b	907.00±276.58b	83.01±2.52a
PM×LA	30.12±5.58a	37.95±7.58a	0.40±0.03a	0.38±0.08a	1 179.75±180.58a	1 288.42±301.29a	79.64±3.22a
PA	28.87±4.28b	70.98±6.35b	0.78±0.06a	1.40±0.06b	1 022.18±258.66b	2 249.64±260.03b	79.33±2.21a
PA×LA	38.17±8.72a	87.44±5.72a	0.73±0.08a	1.61±0.28a	1 274.75±380.28a	2 874.00±139.29a	77.75±3.04a

处理 Treatment	比根长 Specific root length/ (cm·g^?1)	组织密度 Root tissue density/ (g·cm^?3)	比表面积 Specific root surface area/(cm²·g^?1)	根冠比 Root shoot ratio
PM	146.85±5.60b	0.44±0.03a	187.12±7.20b	1.04±0.32a
PM×LA	213.20±10.21a	0.37±0.10a	268.61±12.34a	0.82±0.21b
PA	61.42±3.81a	0.34±0.06a	151.02±4.95a	0.91±0.23a
PA×LA	60.59±6.10a	0.39±0.12a	138.80±6.33b	0.85±0.15a

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Regulation of Lactarius akahatsu on the Growth and Root Metabolites of Pinus massoniana and Pinus armandii Seedlings

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