Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (7): 63-72.doi: 10.11707/j.1001-7488.20220707
• Research papers • Previous Articles Next Articles
Min Li,Xizhou Zhao,Haoyun Wang,Zhongke Lu,Guijie Ding*
Received:
2021-06-27
Online:
2022-07-25
Published:
2022-11-03
Contact:
Guijie Ding
CLC Number:
Min Li, Xizhou Zhao, Haoyun Wang, Zhongke Lu, Guijie Ding. Effects of Drought Stress and Ectomycorrhizal Fungi on the Root Morphology and Exudates of Pinus massoniana Seedlings[J]. Scientia Silvae Sinicae, 2022, 58(7): 63-72.
Table 1
Growth changes of Pinus massoniana seedlings treated with different inoculations under different water conditions"
处理 Treatments | 苗高净增长量 Growth of height/cm | 地径净增长量 Growth of stem diameter/mm | 根生物量 Dry weight of root/(g·plant-1) | 地上生物量 Dry biomass of aboveground/ (g·plant-1) | 总生物量 Dry biomass/(g·plant-1) | 根冠比 Root aboveground ratios | 侵染率 Infection rate (%) |
CKW | 5.13±0.18b | 1.54±0.02b | 0.7±0.05Ab | 4.24±0.43Ab | 4.95±0.94Ab | 0.17±0.02 | 3.17±0.65e |
CKD | 1.93±0.07d | 0.5±0.01d | 0.33±0.04Bb | 1.67±0.14Bb | 2.00±0.38Bb | 0.20±0.02 | 1.07±0.12e |
SpW | 5.63±0.14a | 1.65±0.01a | 1.26±0.12Aa | 5.57±0.66Aa | 6.83±1.54Aa | 0.23±0.01 | 63.98±1.72b |
SpD | 2.13±0.09d | 0.53±0.02d | 0.57±0.08Bab | 2.57±0.60Bab | 3.15±0.63Bab | 0.25±0.08 | 41.39±1.70d |
ScW | 5.67±0.24a | 1.67±0.01a | 1.49±0.21Aa | 6.50±0.98Aa | 7.99±1.07Aa | 0.24±0.04 | 68.52±1.60a |
ScD | 3.17±0.39c | 0.64±0.02c | 0.72±0.13Ba | 3.10±0.42Ba | 3.82±0.44Ba | 0.25±0.06 | 49.26±0.91c |
接菌ECM | < 0.001 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | 0.228 | < 0.001 |
干旱Drought(D) | < 0.001 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | 0.56 | < 0.001 |
ECM×D | < 0.001 | 0.041 | 0.232 | 0.744 | 0.579 | 0.97 | < 0.001 |
Table 2
Root growth differences under different treatments"
处理 Treatments | 根长 Length/cm | 根表面积 Surfale area/cm2 | 平均直径 Avgrage diam/mm | 根体积 Root volume/cm3 | 根尖数 Tips | 分支数 Forks |
CKW | 697.92±12.54b | 105.23±4.87b | 2.23±0.08d | 1.60±0.06c | 6 626.75±466.16c | 12 614.00±353.02c |
CKD | 232.86±18.60cd | 56.80±3.23c | 4.23±0.34c | 0.81±0.08d | 2 168.33±205.85e | 6 643.33±367.73c |
SpW | 1454.13±78.76a | 252.68±16.82a | 7.36±0.61b | 3.62±0.17b | 14 155.33±485.14b | 36 551.00±1 031.20b |
SpD | 248.91±30.44d | 52.65±7.45c | 1.22±0.11e | 0.90±0.07d | 5 789.33±283.58cd | 10 349.00±610.07c |
ScW | 1456.40±116.87a | 251.00±15.92a | 7.77±0.28b | 5.16±0.21a | 27 409.33±2 303.59a | 73 145.33±4 766.79a |
ScD | 490.67±17.46c | 89.57±2.18b | 10.06±0.29a | 1.69±0.14c | 2 991.67±234.52e | 8 395.67±327.10c |
接菌ECM | < 0.001 | < 0.001 | 0.033 | < 0.001 | < 0.001 | < 0.001 |
干旱Drought(D) | < 0.001 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | < 0.001 |
ECM×D | < 0.001 | < 0.001 | < 0.001 | < 0.001 | < 0.001 | < 0.001 |
Table 3
Composition of root exudates"
类别 Category | 种类数Number of kind | |||||||
CK | Sc | Sp | ||||||
浇水Watering | 干旱Drought | 浇水Watering | 干旱Drought | 浇水Watering | 干旱Drought | |||
萜类Terpenoids | 3.65(3) | 4.22(3) | 2.14(3) | 1.40(2) | 5.80(2) | 2.16(2) | ||
酯类Esters | 1.32(2) | 0.54(2) | 1.15(2) | 1.06(2) | 0 | 0.56(1) | ||
醛类Aldehyde | 3.25(4) | 2.47(4) | 1.91(4) | 1.97(4) | 1.42(4) | 3.11(4) | ||
酸类Acids | 86.55(14) | 85.37(15) | 88.26(15) | 84.99(15) | 86.04(14) | 77.67(13) | ||
酮类Ketones | 0.47(1) | 0.56(1) | 0.20(1) | 0.57(1) | 0.78(1) | 1.13(1) | ||
醇类Alcohol | 0.85(1) | 0.71(1) | 0.60(1) | 0.65(1) | 0 | 0 | ||
其他Other | 3.91(3) | 6.12(4) | 5.73(4) | 9.35(6) | 5.96(4) | 15.37(6) | ||
小计(种)Total(Species) | 28 | 30 | 30 | 31 | 25 | 27 |
Table 4
Relative contents of root exudate in different treatments"
类别 Category | 物质名称 Name of substances | 分子式 Molecular formula | 分子量 Molecular weight | 相对含量Relative content (%) | |||||||
CK | Sc | Sp | |||||||||
浇水 Watering | 干旱 Drought | 浇水 Watering | 干旱 Drought | 浇水 Watering | 干旱 Drought | ||||||
萜类Terpenoids | 柠檬烯Limonene | C10H16 | 136 | 0.32 | 0.119 | 0.095 | 0 | 0 | 0 | ||
β-水芹烯β-Phellandrene | C10H16 | 136 | 0.64 | 0.45 | 0.34 | 0.45 | 0.58 | 0.62 | |||
长叶烯Longifolene | C15H24 | 204 | 2.69 | 3.65 | 1.71 | 0.95 | 5.22 | 1.55 | |||
酯类Esters | 丙烯酸-2-乙基己酯 2-Ethylhexyl acrylate | C11H20O2 | 184 | 0.62 | 0.22 | 0.94 | 0.73 | 0 | 0 | ||
癸酸甲酯Methyl caprate | C11H22O2 | 186 | 0.69 | 0.32 | 0.21 | 0.33 | 0 | 0.56 | |||
醛类Aldehyde | 安息香醛Benzaldehyde | C7H6O | 106 | 1.74 | 0.93 | 0.62 | 0.70 | 0.20 | 0.88 | ||
5-甲基糠醛 5-methyl-2- furancarboxaldehyde | C6H6O2 | 110 | 0.23 | 0.15 | 0.07 | 0.10 | 0.15 | 0.22 | |||
3, 5-二甲基苯甲醛 3, 5-dimethyl-benzaldehyde | C9H10O | 134 | 0.79 | 0.39 | 0.79 | 0.56 | 0.48 | 0.40 | |||
香草醛Vanillin | C8H8O3 | 152 | 0.50 | 1.00 | 0.43 | 0.61 | 0.59 | 1.61 | |||
酸类Acids | 丙酸Propanoic acid | C3H6O2 | 74 | 1.70 | 1.14 | 0.20 | 0.31 | 0.03 | 0.19 | ||
2- 甲基丙酸 2-methyl-propanoic acid | C4H8O2 | 88 | 0.36 | 0.57 | 0.06 | 0.13 | 0 | 0 | |||
丁酸Butanoic acid | C4H8O2 | 88 | 0.56 | 0.09 | 0.76 | 0.83 | 0.30 | 0.28 | |||
3-甲基丁酸 3-Methyl-butanoic acid | C5H10O2 | 102 | 0.82 | 1.18 | 0.59 | 0.32 | 0 | 0.47 | |||
丁烯酸Isocrotonic acid | C4H6O2 | 86 | 0.76 | 1.91 | 0.72 | 1.65 | 0.68 | 1.87 | |||
辛酸Octanoic acid | C8H16O2 | 144 | 0 | 0.29 | 0 | 0 | 0.59 | 0 | |||
壬酸Nonanoic acid | C9H18O2 | 158 | 0.19 | 0.28 | 0.37 | 0.19 | 0.50 | 0.40 | |||
(E)-2-辛烯酸 (E)-2-octenoic acid | C8H14O2 | 142 | 0 | 0 | 0.11 | 0.12 | 0 | 0 | |||
正癸酸n-Decanoic acid | C10H20O2 | 172 | 0 | 0 | 0 | 0 | 0.47 | 0 | |||
苯甲酸Benzoic acid | C7H6O2 | 122 | 0.43 | 0.50 | 0.49 | 0.55 | 0.76 | 0.72 | |||
月桂酸Dodecanoic acid | C12H24O2 | 200 | 0.78 | 0.67 | 0.47 | 0.77 | 1.24 | 0.81 | |||
苯乙酸Benzeneacetic acid | C8H8O2 | 136 | 1.35 | 5.79 | 1.27 | 4.13 | 2.04 | 5.99 | |||
十四烷酸Tetradecanoic acid | C14H28O2 | 228 | 1.30 | 2.05 | 2.97 | 3.30 | 9.25 | 12.30 | |||
十五烷酸Pentadecanoic acid | C15H30O2 | 242 | 1.11 | 1.20 | 2.63 | 2.17 | 3.16 | 4.65 | |||
十六烷酸n-hexadecanoic acid | C16H32O2 | 256 | 49.82 | 40.75 | 45.98 | 40.04 | 36.85 | 26.66 | |||
硬脂酸Octadecanoic acid | C18H36O2 | 284 | 25.40 | 23.70 | 26.16 | 24.30 | 20.12 | 14.92 | |||
油酸Oleic acid | C18H34O2 | 282 | 1.97 | 5.25 | 5.52 | 6.19 | 10.07 | 8.40 | |||
醇类Alcohol | 苯甲醇Benzyl alcohol | C7H8O | 108 | 0.85 | 0.71 | 0.60 | 0.65 | 0 | 0 | ||
酮类Ketones | 六氢法尼基丙酮 Hexahydrofarnesyl acetone | C18H36O | 268 | 0.47 | 0.56 | 0.20 | 0.57 | 0.78 | 1.13 |
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