Scientia Silvae Sinicae ›› 2021, Vol. 57 ›› Issue (9): 42-51.doi: 10.11707/j.1001-7488.20210905
Previous Articles Next Articles
Zijing Zhou1,Fuhua Fan1,*,Xianwen Shang1,Huijuan Qin1,Conghui Wang1,Guijie Ding1,Jianhui Tan2
Received:
2021-04-21
Online:
2021-09-25
Published:
2021-11-29
Contact:
Fuhua Fan
CLC Number:
Zijing Zhou,Fuhua Fan,Xianwen Shang,Huijuan Qin,Conghui Wang,Guijie Ding,Jianhui Tan. Effects of Exogenous IAA on Stem Secondary Growth of Pinus massoniana Seedlings[J]. Scientia Silvae Sinicae, 2021, 57(9): 42-51.
Table 1
Primer sequence"
基因 Gene | 序列 Sequence(5′→3′) |
TRINITY_DN10179_c0_g1 | F: TGATGGATGGTGGTTGATAC |
R: CTGATGGGCAAACTGAGATA | |
TRINITY_DN8564_c0_g2 | F: GCGTATTCCGCCAGAGTG |
R: TCCAGAACGAGTGCCCCT | |
TRINITY_DN5190_c1_g1 | F: CAGGCGACTCCACCTACAACT |
R: TGCGGAACACCTAATCCAAAC | |
TRINITY_DN2065_c0_g1 | F: GAACGCAGAGCAATGAAG |
R: AAGCCCACCACTATGACC | |
TRINITY_DN11133_c0_g2 | F: TCACGCTTCCTTTGGCTTTT |
R: CGTACGGTCTGCACTTCATC | |
TRINITY_DN3030_c1_g1 | F: AGAGCAGATTCCAGCAGA |
R: CGGGTTTCCAGTAGATGA | |
TRINITY_DN39456_c0_g1 | F: TCAGCGGACCCTGTTCTA |
R: GACAAAGTTGCCGTGGAG | |
TRINITY_DN40433_c0_g1 | F: AGGGACTGGCTCCTTGTG |
R: CTTCGGAATCGCACCTTT | |
TRINITY_DN99422_c0_g3 | F: GAAGACTTGAAATGCCTGAC |
R: ATAGACCTTGAACGCCTC | |
UBC | F: GATTTATTTCATTGGCAGGC |
R: AGGATCATCAGGATTTGGGT |
Table 2
Lignin, cellulose and hemicellulose contents in stem of P. massoniana seedlings treated with IAA"
IAA | 木质素含量 Lignin content/(mg·g-1) | 纤维素含量 Cellulose content/(mg·g-1) | 半纤维素含量 Hemicellulose content/(mg·g-1) |
CK | 89.95±0.98 a | 199.30±35.81 a | 160.51±1.01 a |
1 mg·L-1 | 116.33±0.07 b | 292.35±18.03 b | 207.13±15.40 b |
50 mg·L-1 | 122.43±3.92 b | 287.18±16.06 b | 202.68±5.75 b |
100 mg·L-1 | 124.94±7.08 b | 285.85±12.47 b | 187.36±3.05 ab |
Table 3
Endogenous hormone contents in stems of P. massoniana seedlings treated with IAA"
内源激素Endogenous hormone/(ng·g-1) | CK | IAA处理 IAA treatment | |
生长素类 Auxins | 吲哚-3-乙酸Indole-3-acetic acid(IAA) | 58.67±1.95 | 113.67±4.91** |
氧化吲哚乙酸Oxidized indoleacetic acid(OxIAA) | 0.00 | 114.00±4.73** | |
吲哚-3-甲醛Indole-3-carboxaldehyde(ICAld) | 4.48±0.04 | 133.00±3.51** | |
吲哚-3-乙酸甲酯Indole-3-methyl acetate(MEIAA) | 3.85±0.10 | 7.18±0.28** | |
吲哚乙酸-亮氨酸Indoleacetic acid-leucine(IAA-Leu) | 1.06±0.08** | 0.56±0.03 | |
吲哚乙酸-天冬氨酸Indoleacetic acid-aspartic acid(IAA-Asp) | 1.89±0.13** | 1.27±0.06 | |
色胺Tryptamine(TRA) | 0.16±0.01 | 0.14±0.00 | |
色氨酸Tryptophan(TRP) | 2 156.67±53.64* | 1 903.33±43.33 | |
3-吲哚丙酸3-Indolepropionic acid(IPA) | 42.63±0.92 | 44.30±2.86 | |
赤霉素类 Gibberellins | 赤霉素1 Gibberellin1(GA1) | 17.67±0.69 | 24.60±2.46 |
赤霉素15 Gibberellin15(GA15) | 0.27±0.05 | 0.27±0.03 | |
油菜素内酯类 Brassinolides | 油菜素内酯Brassinolide(BR) | 0.05±0.00 | 0.09±0.00** |
油菜素甾酮Castasterone(CS) | 19.89±1.18 | 27.03±0.99** | |
28-高油菜素甾酮28-homocastasterone(28-homoCS) | 1.25±0.07 | 1.58±0.03** | |
香蒲甾醇Typhasterol(TY) | 36.38±0.31 | 35.42±0.32 | |
6-脱氧油菜素甾酮6-deoxocastasterone(6-deoxoCS) | 0.02±0.00 | 0.03±0.00 |
Table 4
Statistics of stem transcriptome data of P. massoniana seedlings treated with IAA"
样本 Sample | 原始读数 Raw reads | 清洁读数(清洁读数/原始读数) Clean reads(clean reads/raw reads) | 错误率 Error rate(%) | Q20(%) | Q30(%) | GC含量 GC content(%) |
CK1 | 79 111 552 | 78 288 654(98.96%) | 0.024 4 | 98.31 | 94.73 | 44.47 |
CK2 | 73 641 850 | 72 846 758(98.92%) | 0.024 3 | 98.33 | 94.83 | 44.96 |
CK3 | 86 263 202 | 85 424 670(99.03%) | 0.024 4 | 98.28 | 94.66 | 44.65 |
IAA1 | 87 975 948 | 87 084 030(98.99%) | 0.024 2 | 98.36 | 94.87 | 44.83 |
IAA2 | 93 638 284 | 92 535 608(98.82%) | 0.024 6 | 98.24 | 94.55 | 45.16 |
IAA3 | 77 429 912 | 76 588 978(98.91%) | 0.024 7 | 98.17 | 94.39 | 44.87 |
Table 5
Identification and expression of genes related to secondary growth"
基因号 Gene ID | 注释 Description | 差异倍数 Log2FC | 表达量Expression quantity | |
CK | IAA | |||
激素Hormone | ||||
TRINITY_DN6209_c0_g1 | 油菜素内酯受体激酶Brassinosteroid LRR receptor kinase(BRL1) | 1.92 | 9.15 | 30.11 |
TRINITY_DN105600_c0_g2 | 赤霉素响应蛋白Gibberellin-regulated protein(GASA6) | 1.70 | 2.09 | 6.35 |
木质素Lignin | ||||
TRINITY_DN40433_c0_g1 | 肉桂醇脱氢酶Cinnamyl alcohol dehydrogenases(CAD) | 7.48 | 0 | 1.06 |
纤维素Cellulose | ||||
TRINITY_DN2065_c0_g1 | 纤维素合酶Cellulose synthase(CESA2) | 1.13 | 23.30 | 45.01 |
陈海燕, 刘凯, 余敏, 等. 外源激素IAA和GA3对马尾松应压木形成的影响. 北京林业大学学报, 2015, 37 (5): 134- 139. | |
Chen H Y , Liu K , Yu M , et al. Effect of exogenous IAA and GA3 on formation of compression wood of Pinus massoniana Lamb. Journal of Beijing Forestry University, 2015, 37 (5): 134- 139. | |
丁贵杰, 严仁发, 齐新民. 不同种源马尾松造林效果及经济效益对比分析. 林业科学, 1994, (6): 506- 512. | |
Ding G J , Yan R F , Qi X M . A comparative analysis on planting result and economic benefit of Pinus massoniana Lamb. from different provenance. Scientia Silvae Sinicae, 1994, (6): 506- 512. | |
郭丽玲, 潘萍, 欧阳勋志, 等. 间伐补植对马尾松低效林生长及林分碳密度的短期影响. 西南林业大学学报: 自然科学, 2019, 39 (3): 48- 54. | |
Guo L L , Pan P , Ouyang X Z , et al. Short-term effect of thinning and replanting measures on tree growth and stand carbon density of low-efficiency Pinus massoniana forest. Journal of Southwest Forestry University: Natural Science, 2019, 39 (3): 48- 54. | |
吴修蓉, 周运超, 余星. 镁肥对马尾松幼苗生长与叶片元素积累的影响. 亚热带植物科学, 2019, 48 (1): 11- 16. | |
Wu X R , Zhou Y C , Yu X . Effect of magnesium fertilizer on growth and element accumulation of Pinus massoniana seedlings. Subtropical Plant Science, 2019, 48 (1): 11- 16. | |
张婷, 文晓鹏. 混合接种外生菌根菌对马尾松种子萌发及幼苗生长的影响. 种子, 2016, 35 (10): 1- 5.
doi: 10.3969/j.issn.1000-8071.2016.10.001 |
|
Zhang T , Wen X P . Effect of ectomycorrhizal fungi combination on seed germination and seedling growth of Masson Pine(Pinus massoniana). Seed, 2016, 35 (10): 1- 5.
doi: 10.3969/j.issn.1000-8071.2016.10.001 |
|
周乃富, 张俊佩, 刘昊, 等. 木本植物非均质化组织石蜡切片制作方法. 植物学报, 2018, 53 (5): 653- 660. | |
Zhou N F , Zhang J P , Liu H , et al. New protocols for paraffin sections of heterogeneous tissues of woody plants. Chinese Bulletin of Botany, 2018, 53 (5): 653- 660. | |
Bargmann B O R , Vanneste S , Krouk G , et al. A map of cell type-specific auxin responses. Molecular Systems Biology, 2013, 9, 688.
doi: 10.1038/msb.2013.40 |
|
Ben-Nissan G , Lee J , Borohov A , et al. GIP, a Petunia hybrida GA-induced cysteine-rich protein: a possible role in shoot elongation and transition to flowering. The Plant Journal, 2004, 37 (2): 229- 238.
doi: 10.1046/j.1365-313X.2003.01950.x |
|
Bjorklund S , Antti H , Uddestrand I , et al. Cross-talk between gibberellin and auxin in development of Populus wood: gibberellin stimulates polar auxin transport and has a common transcriptome with auxin. The Plant Journal, 2007, 52 (3): 499- 511.
doi: 10.1111/j.1365-313X.2007.03250.x |
|
Brackmann K , Qi J , Gebert M , et al. Spatial specificity of auxin responses coordinates wood formation. Nature Communications, 2018, 9, 875.
doi: 10.1038/s41467-018-03256-2 |
|
Cano-Delgado A , Yin Y , Yu C , et al. BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis. Development, 2004, 131 (21): 5341- 5351.
doi: 10.1242/dev.01403 |
|
Chaiwanon J , Wang Z . Spatiotemporal brassinosteroid signaling and antagonism with auxin pattern stem cell dynamics in Arabidopsis roots. Current Biology, 2015, 25 (8): 1031- 1042.
doi: 10.1016/j.cub.2015.02.046 |
|
Chen J , Wang L , Immanen J , et al. Differential regulation of auxin and cytokinin during the secondary vascular tissue regeneration in Populus trees. New Phytologist, 2019, 224 (1): 188- 201.
doi: 10.1111/nph.16019 |
|
Fan F, Cui B, Zhang T, et al. 2014. The temporal transcriptomic response of Pinus massoniana seedlings to phosphorus deficiency. PLoS ONE, 9: e1050688. | |
Glweiler L , Uan C , Ller A , et al. Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissues. Science, 1998, 282 (5397): 2226- 2230.
doi: 10.1126/science.282.5397.2226 |
|
Joshi C P , Bhandari S , Ranjan P , et al. Genomics of cellulose biosynthesis in poplars. New Phytology, 2004, 164, 53- 61.
doi: 10.1111/j.1469-8137.2004.01155.x |
|
Li A , Xia T , Xu W , et al. An integrative analysis of four CESA isoforms specific for fiber cellulose production between Gossypium hirsutum and Gossypium barbadense. Planta, 2013, 237 (6): 1585- 1597.
doi: 10.1007/s00425-013-1868-2 |
|
Livak K J , Schmittgen T D . Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△CT method. Methods, 2001, 25 (4): 402- 408.
doi: 10.1006/meth.2001.1262 |
|
Ma D , Xu C , Alejos-Gonzalez F , et al. Overexpression of Artemisia annua cinnamyl alcohol dehydrogenase increases lignin and coumarin and reduces artemisinin and other sesquiterpenes. Frontiers in Plant Science, 2018, 9, 828.
doi: 10.3389/fpls.2018.00828 |
|
Maleki S S , Mohammadi K , Ji K . Characterization of cellulose synthesis in plant cells. The Scientific World Journal, 2016, 2016, 8641373. | |
Maleki S S , Mohammadi K , Movahedi A , et al. Increase in cell wall thickening and biomass production by overexpression of PmCesA2 in poplar. Frontiers in Plant Science, 2020, 11, 110.
doi: 10.3389/fpls.2020.00110 |
|
Mellerowicz E J , Baucher M , Sundberg B , et al. Unravelling cell wall formation in the woody dicot stem. Plant Molecular Biology, 2001, 47 (1/2): 239- 274.
doi: 10.1023/A:1010699919325 |
|
Muller C J , Valdes A E , Wang G , et al. PHABULOSA mediates an auxin signaling loop to regulate vascular patterning in Arabidopsis. Plant Physiology, 2016, 170 (2): 956- 970.
doi: 10.1104/pp.15.01204 |
|
Nairn C J , Haselkorn T . Three loblolly pine CesA genes expressed in developing xylem are orthologous to secondary cell wall CesA genes of angiosperms. The New Phytologist, 2005, 166 (3): 907- 915.
doi: 10.1111/j.1469-8137.2005.01372.x |
|
Nemhauser J L , Mockler T C , Chory J . Interdependency of brassinosteroid and auxin signaling in Arabidopsis. PLoS Biology, 2004, 2 (9): E258.
doi: 10.1371/journal.pbio.0020258 |
|
Nieminen K M , Kauppinen L , Helariutta Y . A weed for wood? Arabidopsis as a genetic model for xylem development. Plant Physiology, 2004, 135 (2): 653- 659.
doi: 10.1104/pp.104.040212 |
|
Pan H , Zhou R , Louie G V , et al. Structural studies of cinnamoyl-CoA reductase and cinnamyl-alcohol dehydrogenase, key enzymes of monolignol biosynthesis. Plant Cell, 2014, 26 (9): 3709- 3727.
doi: 10.1105/tpc.114.127399 |
|
Pan X , Welti R , Wang X . Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry. Nature Protocols, 2010, 5 (6): 986- 992.
doi: 10.1038/nprot.2010.37 |
|
Ponniah S K , Shang Z , Akbudak M A , et al. Down-regulation of hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase, cinnamoyl CoA reductase, and cinnamyl alcohol dehydrogenase leads to lignin reduction in rice(Oryza sativa L. ssp. japonica cv. Nipponbare). Plant Biotechnology Reports, 2017, 11 (1): 17- 27. | |
Sakamoto T , Morinaka Y , Inukai Y , et al. Auxin signal transcription factor regulates expression of the brassinosteroid receptor gene in rice. Plant Journal, 2013, 73 (4): 676- 688.
doi: 10.1111/tpj.12071 |
|
Savidge R A . Auxin and ethylene regulation of diameter growth in trees. Tree Physiology, 1988, 4 (4): 401- 414.
doi: 10.1093/treephys/4.4.401 |
|
Savidge R A . The role of plant hormones in higher plant cellular differentiation Ⅱ: Experiments with the vascular cambium, and sclereid and tracheid differentiation in the pine, Pinus contorta. The Histochemical Journal, 1983, 15 (5): 447- 466.
doi: 10.1007/BF01002699 |
|
Spicer R , Groover A . Evolution of development of vascular cambia and secondary growth. New Phytologist, 2010, 186 (3): 577- 592.
doi: 10.1111/j.1469-8137.2010.03236.x |
|
Taylor B H , Scheuring C F . A molecular marker for lateral root initiation: the RSI-1 gene of tomato(Lycopersicon esculentum Mill) is activated in early lateral root primordia. Molecular General Genetics, 1994, 243 (2): 148- 157.
doi: 10.1007/BF00280311 |
|
Tuominen H , Puech L , Fink S , et al. A radial concentration gradient of indole-3-acetic acid is related to secondary xylem development in hybrid aspen. Plant Physiology, 1997, 115 (2): 577- 585.
doi: 10.1104/pp.115.2.577 |
|
Uggla C , Mellerowicz E J , Sundberg B . Indole-3-acetic acid controls cambial growth in Scots pine by positional signaling. Plant Physiology, 1998, 117 (1): 113- 121.
doi: 10.1104/pp.117.1.113 |
|
Yao X , Remko O . PDK1 regulates auxin transport and Arabidopsis vascular development through AGC1 kinase PAX. Nature Plants, 2020, 6, 544- 555.
doi: 10.1038/s41477-020-0650-2 |
|
Yu M , Liu K , Liu S , et al. Effect of exogenous IAA on tension wood formation by facilitating polar auxin transport and cellulose biosynthesis in hybrid poplar(Populus deltoides×Populus nigra) wood. Holzforschung, 2017, 71 (2): 179- 188.
doi: 10.1515/hf-2016-0078 |
|
Yuan H , Zhao L , Guo W , et al. Exogenous application of phytohormones promotes growth and regulates expression of wood formation-related genes in Populus simonii×P. nigra. International Journal of Molecular Sciences, 2019, 20 (3): 792.
doi: 10.3390/ijms20030792 |
|
Zhao C , Craig J C , Petzold H E , et al. The xylem and phloem transcriptomes from secondary tissues of the Arabidopsis root-hypocotyl. Plant Physiology, 2005, 138 (2): 803- 818.
doi: 10.1104/pp.105.060202 |
|
Zheng L , Gao C , Zhao C , et al. Effects of brassinosteroid associated with auxin and gibberellin on apple tree growth and gene expression patterns. Horticultural Plant Journal, 2019, 5 (3): 93- 108.
doi: 10.1016/j.hpj.2019.04.006 |
|
Zhong C , Xu H , Ye S , et al. Gibberellic acid-stimulated Arabidopsis6 serves as an integrator of gibberellin, abscisic acid, and glucose signaling during seed germination in Arabidopsis. Plant Physiology, 2015a, 169 (3): 2288- 2303. | |
Zhong R , Ye Z . Secondary cell walls: biosynthesis, patterned deposition and transcriptional regulation. Plant and Cell Physiology, 2015b, 56 (2SI): 195- 214. | |
Zhou A , Wang H , Walker J C , et al. BRL1, a leucine-rich repeat receptor-like protein kinase, is functionally redundant with BRI1 in regulating Arabidopsis brassinosteroid signaling. The Plant Journal, 2004, 40 (3): 399- 409.
doi: 10.1111/j.1365-313X.2004.02214.x |
[1] | Chen Xingzhou, Zhou Guoying, Chen Xinggang, Jiang Lingyu, Bao Anhua, Liu Jun. Screening of Effectors of Colletotrichum fructicola in Camellia oleifera [J]. Scientia Silvae Sinicae, 2021, 57(9): 110-120. |
[2] | Peng Xin, Wang Hantang, Guo Chunhui, Yang Zhende, Zhou Jing, Wang Xue, Ding Zhirou. EST-SSR Development and Cryptic Species Identification of the Invasive Gall-Causing Pest Leptocybe invasa (Hymenopetra: Eulophidae) [J]. Scientia Silvae Sinicae, 2021, 57(9): 140-151. |
[3] | Haiyang Wang,Qianli Ma. Adsorption Properties and Mechanisms of Pinus massoniana Bark Nano-Lignocellulose Aerogel Adsorbent for Cr3+/Cu2+/Pb2+/Ni2+ [J]. Scientia Silvae Sinicae, 2021, 57(7): 166-174. |
[4] | Linfeng Ye,Yan Li,Zhongyuan Wang,Shitong Lu,Tiantian Pan,Sen Chen,Jiangbo Xie. Efficiency-Safety Relationships of Hydraulic Conducting System for Branch and Root of Three Pinus Species Growing in Humid Area [J]. Scientia Silvae Sinicae, 2021, 57(7): 194-204. |
[5] | Jinfeng Cai,Xiaoming Yang,Wanwen Yu,Guibin Wang,Fuliang Cao. Development of SSR Molecular Markers Based on Transcriptome Sequencing of Melia azedarach [J]. Scientia Silvae Sinicae, 2021, 57(6): 85-92. |
[6] | Xiaosui Wen,Dunfu Song,Zhongqi Yang,Zhonghui Wang,Mingqing Shi. Relationships between the Emergence of Dastarcus helophoroides (Coleoptera: Bothrideridae) and the Emergence of the Host Monochamus alternatus (Coleoptera: Cerambycidae) in Pinus massoniana Forests [J]. Scientia Silvae Sinicae, 2020, 56(9): 193-200. |
[7] | Yin Wang,Ruiling Yao. Rooting Capacity of Pinus massoniana and the Correlations Endohormones Levels during Subcultur [J]. Scientia Silvae Sinicae, 2020, 56(8): 38-46. |
[8] | Lihua Zhu,Xinyue Zhang,Xinrui Xia,Yu Wan,Shanjun Dai,Jianren Ye. Pathogenicity of Aseptic Bursaphelenchus xylophilus on Pinus massoniana [J]. Scientia Silvae Sinicae, 2020, 56(7): 63-69. |
[9] | Xiaorong Wang,Lei Lei,Tian Fu,Lei Pan,Lixiong Zeng,Wenfa Xiao. Short-Term Effects of Selective Cutting for Tending on Leaf Litter Decomposition Rate and Nutrient Release in Pinus massoniana Forests [J]. Scientia Silvae Sinicae, 2020, 56(4): 12-21. |
[10] | Peihuang Zhu,Yu Chen,Lingzhi Zhu,Rong Li,Kongshu Ji. Codon Usage Bias and Its Influencing Factors in Pinus massoniana Transcriptome [J]. Scientia Silvae Sinicae, 2020, 56(4): 74-81. |
[11] | Xing Wu,Xingfeng Hu,Peizhen Chen,Xiaobo Sun,Fan Wu,Kongshu Ji. Cloning and Functional Analysis of PmPIN1 Gene from Pinus massoniana [J]. Scientia Silvae Sinicae, 2020, 56(3): 184-192. |
[12] | Xiaoli Yan, Wenjia Hu, Yuanfan Ma, yufan Huo, Tuo Wang, Xiangqing Ma. Nitrogen Uptake Preference of Cunninghamia lanceolata, Pinus massoniana, and Schima superba under Heterogeneous Nitrogen Supply Environment and their Root Foraging Strategies [J]. Scientia Silvae Sinicae, 2020, 56(2): 1-11. |
[13] | Kai Sun,Jiasen Wu,Weixing Sheng,Peikun Jiang,Yunqing Zhang,Jiangfei Ge. Potential of Phytolith-Occluded Organic Carbon Sequestration in Masson Pine Stands at Different Ages in Subtropical China [J]. Scientia Silvae Sinicae, 2020, 56(12): 10-18. |
[14] | Xiaoyu Lu,Zhu Chen,Fei Tang,Songling Fu,Jie Ren. Combined Transcriptomic and Metabolomic Analysis Reveals Mechanism of Anthocyanin Changes in Red Maple(Acer rubrum) Leaves [J]. Scientia Silvae Sinicae, 2020, 56(1): 38-53. |
[15] | Liu Wanhui, Chen Feifei, Ye Jianren, Wang Chaoen, Kang Yi, Fu Huanhuan. Identification of Indole-3-Acetamide IAA Synthesis Function and Its Dependent Pathway in Burkholderia pyrrocinia JK-SH007 [J]. Scientia Silvae Sinicae, 2019, 55(9): 121-129. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||