日本松干蚧3个地理种群的遗传分化

doi:10.11707/j.1001-7488.20131213

Abstract

Abstract:

The Japanese pine bast scale, Matsucoccus matsumurae(Hemiptera: Coccoidea: Matsucoccidae)is an important invasive pest in pine forests of China. However, the classification and the population genetic differentiation of this species over various distribution regions have been a problem in the pest management decision-making. In order to provide molecular evidence for the species identification and scientific management of the pest, we collected samples from Fushun in Liaoning Province(LNFS), Qingdao in Shandong Province(SDQD) and Jinhua in Zhejiang Province(ZJJH), respectively, and applied RAPD-PCR technigue with 4 random primers to investigate the genetic variation of the three geographic populations. The results showed that the DNA extracted from individual female adult was enough for RAPD-PCR. The genome size of the species was 9 416 bp. At species level, coefficient of genetic differentiation(G_st)of the population with three replicates was 0.207 8, 0.191 9 and 0.207 5, respectively, indicating that there were 20.78%, 19.19% and 20.75% of genetic diversity resided among populations, and 79.22%, 80.81% and 79.25% of genetic diversity within populations. The minimum genetic distance derived from the three replicates was 0.040 7, 0.035 5 and 0.044 9 between ZJJH and SDQD, respectively, and the maximum ones was 0.065 0, 0.056 2 and 0.067 3 between SDQD and LNFS, which suggested that the three geographic populations would be very close in genetic relationship and that belong to the same species. According to the UPGMA dendrograms, the 3 populations could be grouped into two clades and one contained ZJJH and SDQD and the other contained LNFS only, which indicated a closer genetic relationship between the ZJJH and SDQD populations. This research provides a scientific basis for the pest management and has an important significance in using sex pheromones and the natural enemies of the pine bast scale for biological control in the future.

Key words: pine bast scale, Matsucoccus matsumurae, geographic populations, genetic diversity, RAPD

CLC Number:

S718.7

Yang Qian, Xie Yingping, Fan Jinhua, Shao Shengfu, Wu Jun, Wang Yanshi, Zhao Changsheng, Zhang Yingwei. Genetic Differentiation of Matsucoccus matsumurae from Three Geographic Populations in China[J]. Scientia Silvae Sinicae, 2013, 49(12): 88-96.

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Genetic Differentiation of Matsucoccus matsumurae from Three Geographic Populations in China

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