豫杂一号泡桐二倍体及其同源四倍体的AFLP和MSAP分析

doi:10.11707/j.1001-7488.20131026

Abstract

Abstract:

The DNA base sequences and the methylation of diploid and the corresponding autotetraploid seedlings of Paulownia tomentosa×P. fortunei were investigated with the amplified fragment length polymorphism (AFLP) and the methylation-sensitive amplification polymorphism (MSAP) molecular markers. The result indicated that average 50 to 70 bands were amplified with each pair of AFLP primers and the fragment length was less than 500 bp. The DNA base sequences of diploid P. tomentosa ×P. fortunei were identical to its autotetraploid's. The products of MSAP amplification were from 100 to 500 bp and contained 2 093 and 2 217 restriction sites in the diploid and the corresponding autotetraploid, respectively. Their methylated sites accounted for 36.41% and 39.78% (where fully methylation rate were 12.85% and 14.98%), separately. In the autotetraploid, 21.89% DNA methylation patterns changed compared to its diploid, and the global DNA methylation level was higher than its diploid.

Key words: Paulownia tomentosa×, P. fortunei, diploid, autotetraploid, AFLP, MSAP

CLC Number:

S718.46

Zhang Xiaoshen, Fan Guoqiang, Zhao Zhenli, Cao Xibing, Zhao Gaili, Deng Minjie, Dong Yanpeng. Analysis of Diploid and Its Autotetraploid Paulownia tomentosa×P. fortunei with AFLP and MSAP[J]. Scientia Silvae Sinicae, 2013, 49(10): 167-172.

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Analysis of Diploid and Its Autotetraploid Paulownia tomentosa×P. fortunei with AFLP and MSAP

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