松树脂溃疡病菌及其近缘种AFLP分析*

doi:10.11707/j.1001-7488.20080915

Scientia Silvae Sinicae ›› 2008, Vol. 44 ›› Issue (9): 82-86.doi: 10.11707/j.1001-7488.20080915

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AFLP Analysis of Fusarium circinatum and Relative Species

Liao Tailin^1,2,Ye Jianren¹,Chen Jiandong³

(1. Nanjing Forestry University Nanjing 210037; 2. Kunshan ExitEntry Inspection and Quarantine Bureau of China Kunshan 215301; 3. Jiangsu ExitEntry Inspection and Quarantine Bureau of China Nanjing 210009)

Received:2007-10-08 Revised:1900-01-01 Online:2008-09-25 Published:2008-09-25

Abstract

Abstract:

AFLP fingerprinting analysis of Fusarium circinatum Nirenberg and O'Donnell and relative species was carried out. Ten primer paris that could generated abundant polymorphism fragments were screened out. Total 298 nucleotide acid fragments were amplified with the primers from the template of the seventeen strains of Fusarium spp., DNA and among\|them 283 fragments among were polymorphic. Percentage of polymorphism fragments produced by each pair of AFLP primer pair was 94.97% in average, and varied from 89.29%～100%. All these data indicated that considerable genetic variation existed among F. circinatum and relative species at DNA level. Molecular genetic distances among Fusariumspp. were calculated, and the relationship among them was described quantitatively. Compared with biological species. The result of cluster analysis were basically similar to the phenotypic classification of species. Analysis of genetic diversity of E\|AT/M\|CAA AFLP fingerprinting of Fusarium spp. detormined, unique and different bands for each species and all Fusariumsection Liseolatested were identified based on the E AT/ M CAA AFLP fingerprinting.

Key words: Fusarium circinatum, AFLP, genetic relationship, identification

Liao Tailin;Ye Jianren;Chen Jiandong. AFLP Analysis of Fusarium circinatum and Relative Species[J]. Scientia Silvae Sinicae, 2008, 44(9): 82-86.

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AFLP Analysis of Fusarium circinatum and Relative Species

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