柳树种质资源遗传多样性和亲缘关系的CE-AFLP分析

doi:10.11707/j.1001-7488.20130606

Abstract

Abstract:

Genetic diversity and genetic relationship of 37 Salix germplasms from domestic and foreign were analysed using the CE-AFLP(capillary electrophoresis-amplified fragment length polymorphism)technique. The primer combinations of EcoRI+3/MseI+3 and PstI+2/MseI+3 were used in this study. The result indicated that the 11 primer pairs were used to generate 1 580 fragments, of which 1 399 fragments were polymorphic. The polymorphism rate varied from 80.65% to 94.67%, showing a high genetic diversity among Salix. NTSYS-pc 2.10e software was used for cluster analysis and principal component analysis. The 37 germplasms were divided into two major categories with the similarity coefficient of 0.63, and one belonging to arbor Salix and others to the shrubs except for S. paraplesia. Cluster analysis and principal component analysis were generally consistent with traditional taxonomy. However, there was obvious difference in the genome level between S. suchowensis and S. psammophi in the section Helix. This study on the genetic diversity and genetic relationship of some Salix germplasms at the DNA level would provide a theoretical basis for Salix molecular classification, germplasm resources conservation and utilization, and variety improvement.

Key words: Salix, genetic diversity, genetic relationship, CE-AFLP

CLC Number:

Jia Huixia, Wu Lishuan, Hu Jianjun, Lu Mengzhu. Genetic Diversity and Genetic Relationship of Salix Germplasms Revealed by CE-AFLP Analysis[J]. Scientia Silvae Sinicae, 2013, 49(6): 37-44.

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Genetic Diversity and Genetic Relationship of Salix Germplasms Revealed by CE-AFLP Analysis

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