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Scientia Silvae Sinicae ›› 2014, Vol. 50 ›› Issue (3): 99-108.doi: 10.11707/j.1001-7488.20140314

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Effect of Methyl Methanesulphonate on DNA Methylation of Witches’Broom Seedlings of Paulownia tomentosa

Cao Xibing, Zhao Zhenli, Fan Guoqiang, Deng Minjie, Dong Yanpeng   

  1. Institute of Paulownia, Henan Agricultural University Zhengzhou 450002
  • Received:2013-08-14 Revised:2013-11-29 Online:2014-03-25 Published:2014-04-16
  • Contact: 范国强

Abstract:

Changes of the genomic DNA sequences and DNA methylation of P. tomentosa witches' broom seedlings treated with MMS and the specific DNA methylation fragments were analyzed using amplified fragment length polymorphism (AFLP) and methylation sensitive amplification polymorphism (MSAP), respectively. The results showed that there was no difference in DNA base sequences between the healthy seedlings and MMS-treated seedlings at AFLP level. The DNA methylation level of the witches' broom seedlings gradually increased with the increase of MMS concentration, but DNA methylation level of the seedlings treated with the highest concentration (100 mg·L-1)of MMS was still lower than that of the healthy one. Compared with the witches' broom seedlings, DNA demethylation polymorphisms of the MMS-treated seedlings were lower than those of DNA methylation polymorphisms. The specific fragments caused by DNA methylation would be involved in substance and energy metabolism,transcription and transportation, signal transduction and pathogenic factor. Thus the witches' broom was resulted from the decreases of DNA methylation levels and DNA methylation pattern changes of P. tomentosa.

Key words: Paulownia tomentosa, witches' broom, methyl methanesulphonate(MMS), DNA methylation, AFLP, MSAP

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