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Scientia Silvae Sinicae ›› 2012, Vol. 48 ›› Issue (2): 63-68.doi: 10.11707/j.1001-7488.20120209

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Phenotypic Observation and Analysis of Inflorescence Variation of Autotetraploid Robinia pseudoacacia

Lu Chao1, Cui Binbin1,2, Huang Lujun1, Sun Peng1, Zhang Guojun1,3, Li Yun1   

  1. 1. National Engineering Laboratory for Tree Breeding College of Biological Sciences and Biotechnology, Beijing Forestry University Beijing 100083;2. Department of Biology and Chemistry, Baoding University Baoding 071000;3. College of Horticulture Science and Technology, Hebei Normal University of Science & Technology Qinhuangdao 066004
  • Received:2011-06-09 Revised:2011-08-25 Online:2012-02-25 Published:2012-02-25

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Abstract:

A comparative study between autotetraploid and diploid Robinia pseudoacacia was carried out to investigate the mechanism of genetic variations of inflorescence in autotetraploid. The result showed there were no significantly differences in the length of inflorescence between autotetraploid and diploid R. pseudoacacia, but the number of florets was significantly more in autotetraploid than the diploid. The florets of the autotetraploid R. pseudoacacia in different positions showed the remarkable asynchrony. DNA sequences of homologous genes of LFY and TFL 1 were cloned from autotetraploid and diploid, and their homology reached to 98.44% and 98.00%, respectively. The treatment with the acazC could observably reduce the inflorescence variation and promote inflorescence bloom prematurely.

Key words: Robinia pseudoacacia, autotetraploid, variation of inflorescence, methylation, floral meristem identity genes

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