橡胶树冷应答转录组cDNA-AFLP分析

doi:10.11707/j.1001-7488.20100310

Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (3): 62-67.doi: 10.11707/j.1001-7488.20100310

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cDNA-AFLP Analysis on Transcriptomics of Hevea brasiliensis Induced by Cold Stress

An Zewei^1,²,Chen Genhui³,Cheng Han¹,Zhao Yanhong⁴,Xie Lili^1,² Huang Huasun¹

1.State Center for Rubber BreedingRubber Research Institute, Chinese Academy of Tropical Agricultural SciencesDanzhou 571737;2.College of Agriculture, Hainan UniversityHaikou 570228; 3. Longyan Institute of Agricultural Sciences, Fujian ProvinceLongyan 364000;4.College of Life Sciences, Ludong UniversityYantai 264025

Received:2008-12-18 Revised:1900-01-01 Online:2010-03-25 Published:2010-03-25

Abstract

Abstract:

Rubber tree (Hevea brasiliensis) is the main source of nature rubber. Although rubber planting industry has become one of the important industries of Chinese agriculture in tropical areas, low temperature stress still restricts the development of rubber planting industry in China. The differential transcription of 11 cold-resistant rubber clones and 12 cold susceptible rubber clones was analyzed with and without cold stress by cDNA-AFLP. Through screening 100 primer sets, a total of 12 specific fragments were identified, cloned and sequenced, whose GenBank accession No. was GO269543-GO269554 respectively. By aligning with BLASTn and BLASTx, three fragments, TDF7-8, TDF5-15 and TDF16-10, exhibited the high homology with the resistance related gene ABC transport protein family, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DHAPS) and WRKY genes, respectively. All of the three fragments were up-regulated under cold stress. The other 9 fragments didn’t have high homologous sequences in GenBank. The results would be useful knowledge for rubber cold-resistant molecular breeding in future.

Key words: Hevea brasiliensis, cDNA-AFLP, abiotic stress, cold acclimation, molecular breeding

An Zewei;Chen Genhui;Cheng Han;Zhao Yanhong;Xie Lili;Huang Huasun. cDNA-AFLP Analysis on Transcriptomics of Hevea brasiliensis Induced by Cold Stress[J]. Scientia Silvae Sinicae, 2010, 46(3): 62-67.

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cDNA-AFLP Analysis on Transcriptomics of Hevea brasiliensis Induced by Cold Stress

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