部分箬竹属植物的荧光AFLP分析*

doi:10.11707/j.1001-7488.20091106

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (11): 32-35.doi: 10.11707/j.1001-7488.20091106

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Genetic Diversity of Indocalamus Determined by AFLP Marker

Mu Shaohua¹, Peng Zhenhua², Sun Qixiang², Gao Zhimin², Li Xueping¹

1. Key Laboratory of Bamboo and Rattan Science and Technology of State Forestry Administration International Center for Bamboo and Rattan Beijing 100102;2. Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091

Received:2008-08-28 Revised:1900-01-01 Online:2009-11-25 Published:2009-11-25
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Abstract

Abstract:

Amplified fragment length polymorphism (AFLP) was used to study DNA diversity of 16 species in Indocalamus and other 5 similar bamboo species. Genomic DNA was digested with EcoRⅠ and MseⅠ enzymes and amplified with 9 (EcoRⅠ+3)／(MseⅠ+3)　primer combinations. In the 21 samples, 9 EcoRⅠ-MseⅠ AFLP primer combinations produced 1 367 legible bands, of which there were 115 common bands, 273 specific bands and 72 specific missing bands. In the 16 samples of Indocalamus, 1 193 legible bands were obtained by using 9 primer combinations，with 190 common bands, 177 specific bands and 98 specific missing bands. All the specific bands and specific missing bands would be used as molecular markers to identify these species. The AFLP markers indicated that I. Migoi and I. Latifolius were the same species.

Key words: Indocalamus, AFLP, diversity

CLC Number:

Mu Shaohua;Peng Zhenhua;Sun Qixiang;Gao Zhimin;Li Xueping. Genetic Diversity of Indocalamus Determined by AFLP Marker[J]. Scientia Silvae Sinicae, 2009, 12(11): 32-35.

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Genetic Diversity of Indocalamus Determined by AFLP Marker

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