杉木第2代育种群体优良基因型的RAPD遗传变异(英文)

doi:10.11707/j.1001-7488.20071209

Scientia Silvae Sinicae ›› 2007, Vol. 43 ›› Issue (12): 50-55.doi: 10.11707/j.1001-7488.20071209

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Molecular Characterization of Elite Genotypes within a Second-Generation Chinese Fir (Cunninghamia lanceolata) Breeding Population Using RAPD Markers

Li Mei^1,2,Shi Jisen²,Li Fagen¹,Gan Siming¹

1.Research Institute of Tropical Forestry,CAF Guangzhou 510520; 2.Key Laboratory of Forest Tree Genetics and Gene Engineering of State Forestry Administration Nanjing Forestry University Nanjing 210037

Received:2007-05-11 Revised:1900-01-01 Online:2007-12-25 Published:2007-12-25

Abstract

Abstract:

RAPD markers were used to characterize the genetic diversity and relationships among 182 elite genotypes selected from second-generation trials as breeds for advanced generation breeding in Chinese Fir (Cunninghamia lanceolata). Twenty-nine 10-mer primers generated a total of 311 fragments,245 (78.8%) of which were polymorphic. Cluster analyses allowed for all 182 genotypes to be grouped into 11 clusters. AMOVA analyses showed that a high proportion (96.8%) of total genetic diversity resided within the field trials from which the elite genotypes were selected,whereas genetic diversity among field trials was low but significant (Φ_st =0.032,P<0.001). These results demonstrate that high levels of genetic diversity exist within the second-generation breeding population of Chinese Fir and hence suggest that there is good potential for genetic gains from further breeding.

Key words: Chinese Fir (Cunninghamia lanceolata), elite genotype, genetic diversity, RAPD

Li Mei;Shi Jisen;Li Fagen;Gan Siming. Molecular Characterization of Elite Genotypes within a Second-Generation Chinese Fir (Cunninghamia lanceolata) Breeding Population Using RAPD Markers[J]. Scientia Silvae Sinicae, 2007, 43(12): 50-55.

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Molecular Characterization of Elite Genotypes within a Second-Generation Chinese Fir (Cunninghamia lanceolata) Breeding Population Using RAPD Markers

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