林业科学 ›› 2022, Vol. 58 ›› Issue (8): 136-148.doi: 10.11707/j.1001-7488.20220814
李志,薛晓焱,刘震,蔡齐飞,耿晓东,冯建,周慧娜,张涛,李明婉,王艳梅*
收稿日期:
2021-12-09
出版日期:
2022-08-25
发布日期:
2022-12-19
通讯作者:
王艳梅
基金资助:
Zhi Li,Xiaoyan Xue,Zhen Liu,Qifei Cai,Xiaodong Geng,Jian Feng,Huina Zhou,Tao Zhang,Mingwan Li,Yanmei Wang*
Received:
2021-12-09
Online:
2022-08-25
Published:
2022-12-19
Contact:
Yanmei Wang
摘要:
目的: 探明健康和感病山桐子植株土壤、树皮及叶片细菌群落结构、多样性和功能特征,为致病菌验证及山桐子病害生物防控提供参考。方法: 基于高通量测序技术,通过OTU数量、鉴定类群、多样性指数及功能预测等方法,分析健康和感病山桐子不同器官区细菌组成、多样性和功能的差异。结果: 测序结果表明,山桐子的不同器官区都有其各自特有的菌群,变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)是土壤主要的细菌类群,蓝细菌门(Cyanobacteria)、变形菌门是树皮和叶片主要的细菌类群;健康和感病山桐子相同器官区的优势菌属也存在差异,感病山桐子树皮中肠杆菌科的Brenneria和Hafnia-Obesumbacterium相对丰度显著高于健康山桐子;多样性分析结果显示,山桐子各器官区细菌群落多样性和丰富度由高到低均表现为土壤>树皮>叶片,土壤和叶片细菌群落Chao1指数、Shannon指数和Simpson指数在健康植株和病株间无显著差异,感病树皮细菌群落Simpson指数显著高于健康树皮;PICRUSt功能预测表明,感病山桐子根际土壤相关生物合成功能基因富集,感病山桐子树皮金黄色葡萄球菌感染及相关氨基酸代谢功能基因富集。结论: 山桐子植株感病后,各器官区细菌群落结构和多样性均受到影响,叶片区域细菌群落结构受影响较小,根际土壤、树皮区域细菌的群落结构和功能存在显著差异,感病植株树皮区域细菌群落多样性升高,感病树皮样本中存在丰度较高的林木病原细菌,说明山桐子主要发病区域为植株树皮区域。
中图分类号:
李志,薛晓焱,刘震,蔡齐飞,耿晓东,冯建,周慧娜,张涛,李明婉,王艳梅. 山桐子健康和感病植株不同器官区细菌群落结构、多样性与功能预测分析[J]. 林业科学, 2022, 58(8): 136-148.
Zhi Li,Xiaoyan Xue,Zhen Liu,Qifei Cai,Xiaodong Geng,Jian Feng,Huina Zhou,Tao Zhang,Mingwan Li,Yanmei Wang. Analysis of Bacterial Community Structure, Diversity and Functional Prediction in Different Organs of Healthy and Diseased Idesia polycarpa Plants[J]. Scientia Silvae Sinicae, 2022, 58(8): 136-148.
表1
不同器官区细菌Alpha多样性指数①"
微生物 Microbial | 处理 Treatment | Chao1指数 Chao1 | 香农指数 Shannon | 辛普森指数 Simpson | 覆盖度 Goods coverage |
细菌 Bacteria | G | 6 435.47±664.00a | 11.79±0.14a | 0.999±0.000 07a | 0.983±0.009 7b |
BG | 6 718.15±507.47a | 11.57±0.32a | 0.998±0.000 53a | 0.973±0.002 6c | |
FG | 6 358.99±447.41a | 11.67±0.11a | 0.999±0.000 02a | 0.972±0.003 4c | |
P | 427.49±116.28b | 3.06±0.43b | 0.612±0.042 84c | 0.999±0.000 5a | |
BP | 414.14±125.19b | 3.50±0.86b | 0.752±0.084 34b | 0.998±0.000 6a | |
Y | 117.78±34.15b | 0.79±0.06c | 0.268±0.053 04d | 0.999±0.000 3a | |
BY | 90.19±3.42b | 0.63±0.16c | 0.208±0.089 40d | 0.999±0.000 1a |
表2
KEGG数据库主要代谢通路丰度"
代谢通路 Metabolic pathway | 丰度 Abundance | |||||||
一级 Level 1 | 二级 Level 2 | G | BG | P | BP | Y | BY | |
细胞进程 Cellular processes | 细胞生长和死亡 Cell growth and death | 587.17 | 578.99 | 564.33 | 498.48 | 567.72 | 568.1 | |
细胞运动 Cell motility | 964.57 | 918.76 | 631.46 | 987.7 | 242.87 | 236.61 | ||
环境信息处理 Environmental information processing | 膜运输 Membrane transport | 702.54 | 717.12 | 656.2 | 840.38 | 606.78 | 604.78 | |
遗传信息处理 Genetic information processing | 折叠、分类和降解 Folding, sorting and degradation | 1 368.45 | 1 346.78 | 1 474.85 | 1 196.81 | 1 642.69 | 1 647.76 | |
复制和修复 Replication and repair | 2 132.13 | 2 084.86 | 1 804.58 | 1 722.23 | 1 796.45 | 1 797.94 | ||
翻译 Translation | 1 147.08 | 1 123.26 | 1 085.9 | 913.41 | 1 185.56 | 1 188.41 | ||
代谢 Metabolism | 氨基酸代谢 Amino acid metabolism | 5 794.02 | 5 726.99 | 4 552.88 | 4 328.21 | 4 303.87 | 4 296.93 | |
其他次生代谢产物的生物合成 Biosynthesis of other secondary metabolites | 1 271.03 | 1 268.49 | 821.18 | 741.07 | 791.38 | 804.33 | ||
碳水化合物代谢 Carbohydrate metabolism | 5 840.9 | 5 829.34 | 4 711.6 | 4 792.86 | 4 444.7 | 4 440.85 | ||
能量代谢 Energy metabolism | 2 221.1 | 2 199.72 | 3 034.14 | 2 390.84 | 3 500.67 | 3 513.25 | ||
聚糖生物合成与代谢 Glycan biosynthesis and metabolism | 1 158.34 | 1 188.84 | 1 062.5 | 952.41 | 1 082.64 | 1 082.92 | ||
脂质代谢 Lipid metabolism | 3 215.01 | 3 132.09 | 1 923.35 | 2 065.19 | 1 501.93 | 1 506.45 | ||
辅助因子和维生素的代谢 Metabolism of cofactors and vitamins | 5 058.61 | 4 993.13 | 5 618.58 | 4 713.01 | 6 123.86 | 6 123.65 | ||
其他氨基酸代谢 Metabolism of other amino acids | 3 477.05 | 3 464.45 | 3 380.19 | 3 187.17 | 3 329.89 | 3 333.09 | ||
萜类化合物和聚酮类化合物的代谢 Metabolism of terpenoids and polyketides | 4 257.25 | 4 239.98 | 2 950.06 | 2 943.4 | 2 651.67 | 2 593.58 | ||
核苷酸代谢 Nucleotide metabolism | 668.77 | 659.69 | 657.54 | 605.25 | 688.55 | 689.81 | ||
外源生物降解与代谢 Xenobiotics biodegradation and metabolism | 3 432.16 | 3 166.33 | 2 303.41 | 2 555.65 | 1 674.51 | 1 657.13 |
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