SA对低温胁迫后枇杷幼果AsA-GSH循环酶系统的影响

doi:10.11707/j.1001-7488.20110907

林业科学 ›› 2011, Vol. 47 ›› Issue (9): 36-42.doi: 10.11707/j.1001-7488.20110907

SA对低温胁迫后枇杷幼果AsA-GSH循环酶系统的影响

黄志明, 吴锦程, 陈伟健, 蔡丽琴, 谢翠萍, 林良津, 黄世杰, 叶美兰

莆田学院环境与生命科学系莆田 351100

收稿日期:2010-09-27 修回日期:2010-12-14 出版日期:2011-09-25 发布日期:2011-09-25
通讯作者: 吴锦程

Effects of SA on Enzymes of Ascorbate-glutathione Cycle in Young Loquat Fruits after Low Temperature Stress

Huang Zhiming, Wu Jincheng, Chen Weijian, Cai Liqin, Xie Cuiping, Lin Liangjin, Huang Shijie, Ye Meilan

Environment and Life Science Department of Putian University Putian 351100

Received:2010-09-27 Revised:2010-12-14 Online:2011-09-25 Published:2011-09-25

摘要/Abstract

摘要：

研究水杨酸处理对"早钟6号"枇杷幼果AsA-GSH循环酶系统的影响,探讨SA对枇杷幼果抗寒性的作用机理。结果表明: 采用40和70 mg·L^-1的SA处理可提高低温胁迫后枇杷幼果还原型谷胱甘肽(GSH)含量,降低氧化型谷胱甘肽(GSSG)含量,导致GSH/GSSG比率的上升,同时也提高低温胁迫后枇杷幼果谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GPX)和谷胱甘肽-S-转移酶(GST)的活性,启动细胞的解毒功能,从而降解低温胁迫后所产生的过量过氧化产物。此外,SA处理还可提高低温胁迫后枇杷幼果抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)和单脱氢抗坏血酸还原酶(MDAR)活性以及还原型抗坏血酸(AsA)含量, 降低氧化型抗坏血酸(DHA)、过氧化氢(H₂O₂)和丙二醛(MDA)含量以及相对电导率(REC),导致AsA/DHA比率的上升,提高枇杷幼果组织内部的抗氧化能力,从而起到保护作用。SA处理可提高低温胁迫后枇杷幼果的抗氧化性, 增强其抗寒能力,其中以70 mg·L^-1的SA处理效果较好。

关键词: 枇杷幼果, 水杨酸, 低温胁迫, AsA-GSH循环

Abstract:

A study on effects of salicylic acid (SA) treatment on enzymes of ascorbate-glutathione cycle in young loquat (Eriobotrya japonica cv.'Zaozhong No.6’) fruits was conducted to explore the mechanism of SA in promoting the chill-resistance of young loquat fruits to low temperature. Results indicated that 40 and 70 mg·L^-1 SA treatments increased the reduced glutathione (GSH) contents, and decreased the oxidized glutathione (GSSG) content. Therefore, the treatment resulted in elevating the ratio of GSH/GSSG in young loquat fruits under low temperature stress. Meanwhile, SA treatment also promoted the activities of GSH-dependent glutathione reductase (GR), glutathione-S-transferases (GST), and glutathione peroxidase (GPX) in young loquat fruits, which could activate its cell detoxification function by degrading peroxides caused by low temperature stress. Furthermore, SA treatment increased ascorbate peroxidase (APX), dehydroascorbate reductase(DHAR), monodehydroascorbate reductase (MDAR) activities and ascorbate (AsA) contents,and decreased the contents of dehydroascorbate (DHA), super hydrogen (H₂O₂), malondialdehyde (MDA) contents and relative electric conductivity (REC), which led to an increase of AsA/DHA ratio, and ultimately resulted in alleviating the oxidative damage caused by low temperature stress. It is concluded that SA could significantly improve oxidation resistance and chill-resistance of young loquat fruits. SA concentration at 70 mg·L^-1 was preferable for young loquat fruits.

Key words: young loquat fruit, salicylic acid, low temperature stress, ascorbate-glutathione cycle

中图分类号:

Q948.1

黄志明;吴锦程;陈伟健;蔡丽琴;谢翠萍;林良津;黄世杰;叶美兰. SA对低温胁迫后枇杷幼果AsA-GSH循环酶系统的影响[J]. 林业科学, 2011, 47(9): 36-42.

Huang Zhiming;Wu Jincheng;Chen Weijian;Cai Liqin;Xie Cuiping;Lin Liangjin;Huang Shijie;Ye Meilan. Effects of SA on Enzymes of Ascorbate-glutathione Cycle in Young Loquat Fruits after Low Temperature Stress[J]. Scientia Silvae Sinicae, 2011, 47(9): 36-42.

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SA对低温胁迫后枇杷幼果AsA-GSH循环酶系统的影响

Effects of SA on Enzymes of Ascorbate-glutathione Cycle in Young Loquat Fruits after Low Temperature Stress

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