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›› 2013, Vol. 49 ›› Issue (1): 107-113.doi: 10.11707/j.1001-7488.20130116

• 论文 • 上一篇    下一篇

SA及H2O2在杨树与溃疡病菌互作中的表达差异

马健1, 刘振宇2, 吕全1, 梁军1, 严东辉1, 张星耀1   

  1. 1. 中国林业科学研究院森林生态环境与保护研究所 国家林业局森林生态学与森林保护学重点实验室 北京 100091;2. 山东农业大学植物保护学院 泰安 271018
  • 收稿日期:2011-12-20 修回日期:2012-08-10 出版日期:2013-01-25 发布日期:2013-01-25
  • 通讯作者: 严东辉

Differential Expression of SA and H2O2 between Susceptible and Resistant Poplars Challenged by Botryosphaeria dothidea

Ma Jian1, Liu Zhenyu2, Lü Quan1, Liang Jun1, Yan Donghui1, Zhang Xingyao1   

  1. 1. Research Institute of Forest Ecology, Environment and Protection, CAF Key Lab of Forest Ecology and Forest Protection of State Forestry Administration Beijing 100091;2. College of Plant Protection, Shandong Agricultural University Tai'an 271018
  • Received:2011-12-20 Revised:2012-08-10 Online:2013-01-25 Published:2013-01-25

摘要: 以毛白杨和北京杨为试验材料,研究不同杨树接种溃疡病菌后信号分子水杨酸(SA)和过氧化氢(H2O2)的变化差异。结果表明: 毛白杨接种后SA与H2O2含量显著上升,72 h时内源游离SA含量达到峰值(191.41 μg·g-1 FW),并伴随H2O2含量的增加和CAT酶活性的降低;而北京杨接种后内源游离SA含量变化不显著(峰值仅为7.83 μg·g-1 FW),不伴随CAT活性的下降,且H2O2含量与毛白杨相比达到峰值的时间早,峰值低(分别为737.52和306.99 nmol·g-1 FW),说明不同杨树与溃疡病菌互作体系中SA与H2O2差异显著;同时,克隆获得编码毛白杨水杨酸结合蛋白PtSABP和北京杨PbSABP蛋白的2个基因,氨基酸序列比对分析证明,PtSABPPbSABP编码的氨基酸与烟草(NtSABP)氨基酸同一性为90%。RT-PCR分析表明: 接种后毛白杨SABP基因的表达在6~24 h时受到抑制,推测此时SA与SABP结合,抑制SABP的CAT活性,从而促使24 h后H2O2含量的显著升高。本试验证明:不同杨树接种 溃疡病菌后SA与H2O2变化差异显著,杨树对溃疡病的抗性与其体内SA与H2O2的积累情况有关。

关键词: 杨树, 溃疡病菌, 水杨酸, 过氧化氢

Abstract: Variations in salicylic acid (SA) and hydrogen peroxide (H2O2) were analyzed in different resistance poplars (Populus tomentosa and Populus × beijingensis) inoculated with Botryosphaeria dothidea. The results showed that the SA content increased in P. tomentosa, the concentration of endogenous free SA peaked (191.41 μg·g-1 FW) at 72 h, which was accompanied with the reduction of CAT activity and increase in H2O2 content. However, in P. X beijingensis the SA content did not significantly arose (7.83 μg·g-1 FW), and was not accompanied by decreases in CAT activity and H2O2 content. The H2O2 peak occurred earlier with lower values compared with P. tomentosa (306.99 nmol·g-1 FW vs 737.52 nmol·g-1 FW). The above results indicated there were significant differences in SA and H2O2 in different poplars interacted with B. dothidea. Meanwhile, we isolated and identified full-length coding sequence of PtSABP and PbSABP from P. tomentosa and P. × beijingensis stem cDNAs. The proteins of PtSABP and PbSABP belong to the Catalase_like superfamily, and their sequences are 90% identical to that of tobacco SABP at the amino acid level based on NCBI blast and phylogenetic tree analysis. RT-PCR analysis showed that gene expression of CAT was inhibited in 6~24 h after inoculated with B. dothidea, suggesting that SA was bound with SABP at this time, inhibiting CAT activity, thus promoting significant increase in H2O2 content at 24 h after inoculation. The experiments determined that the difference of content of SA and H2O2 in poplars was due to inoculated with B. dothidea, SABP was involved in the disease resistance of poplar, and suggested that the resistance of poplars to B. dothidea would be relevant to the accumulation of SA and H2O2.

Key words: Populus spp., Botryosphaeria dothidea, salicylic acid(SA), H2O2

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