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林业科学 ›› 2005, Vol. 41 ›› Issue (3): 54-57.doi: 10.11707/j.1001-7488.20050309

• 论文及研究报告 • 上一篇    下一篇

低温和PEG“渗控”预处理促进石楠种子萌发的研究

郑淮兵1 董丽1 郑彩霞2   

  1. 1.北京林业大学园林学院,北京100083;2.北京林业大学生物科学与技术学院,北京100083
  • 收稿日期:2003-06-02 修回日期:1900-01-01 出版日期:2005-05-25 发布日期:2005-05-25

Improving Germination of Photinia Seed by Low Temperature and PEG Pretreatment

Zheng Huaibing1,Dong Li1,Zheng Caixia2   

  1. 1.College of Landscape and Architecture,Beijing Forestry University Beijing100083; 2.College of Biological Science and Technology, Beijing Forestry University Beijing100083
  • Received:2003-06-02 Revised:1900-01-01 Online:2005-05-25 Published:2005-05-25

摘要:

用0~-2℃低温和不同水平的PEG预处理石楠种子,可提高种子的活力指数,增加可溶性蛋白、煮沸稳定蛋白的含量,提高SOD、CAT活性,降低MDA含量与POD活性;SDS PAG电泳图谱表明:15%PEG预处理的种子,在萌发的第4天,分子量分别为40.27、42.5、44.5、46.2、50.8kD的5条多肽带的染色比未处理种子的深。

关键词: 石楠, 种子预处理, 低温胁迫, PEG

Abstract:

The effects of low temperature and PEG pretreatment on germination of Photinia seeds were investigated.During the germination of seeds pretreated with cold stress at 0~-2 ℃ for 24 h,the physiological and biochemical changes of the seeds were examined.In the PEG-pretreated seeds,the seed vigor index,the content of soluble proteins,boiling-stable proteins,as well as the activity of superoxide dismutase(SOD) and catalase (CAT) increased while the MDA content and the activity of peroxidase (POD) decreased.Meanwhile,the color of bands of polypeptides of 40.27、42.5、44.5、46.2、50.8kDwere darker in 15% PEG-pretreated seeds after 4 days germination at 21 ℃

Key words: Photinia, pretreated_seed, cold stress, PEG