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林业科学 ›› 2011, Vol. 47 ›› Issue (9): 33-35.doi: 10.11707/j.1001-7488.20110906

• 论文 • 上一篇    下一篇

异叶苦竹胚和胚乳的发育及淀粉粒动态

林树燕, 刘国华, 丁雨龙   

  1. 南京林业大学竹类研究所 南京210037
  • 收稿日期:2010-05-11 修回日期:2011-02-25 出版日期:2011-09-25 发布日期:2011-09-25
  • 通讯作者: 丁雨龙

Accumulation Dynamics of Starch Grains and the Development of Embryo and Endosperm in Arundinaria simonii f. heterophyllus

Lin Shuyan, Liu Guohua, Ding Yulong   

  1. Bamboo Research Institute of NanJing Forestry University NanJing 210037
  • Received:2010-05-11 Revised:2011-02-25 Online:2011-09-25 Published:2011-09-25

摘要:

采用常规石蜡切片法,观察异叶苦竹胚和胚乳的发育过程,部分切片用PAS染色观察胚乳发育过程中的淀粉积累分布动态。结果表明: 异叶苦竹合子的休眠期为5天,胚的发育经过二细胞原胚、多细胞原胚、梨形原胚、分化胚和成熟胚阶段,发育类型为禾本型,成熟胚具有胚根、胚芽、盾片、胚芽鞘、胚根鞘、外胚叶等典型禾本科植物成熟胚的结构。胚乳发育包括游离核、细胞化和生长成熟3个阶段,发育类型为核型。授粉后16天左右,淀粉粒开始在细胞核周围积累,以后逐渐增多,充满整个胚乳。

关键词: 异叶苦竹, 胚, 胚乳, PAS反应

Abstract:

The development of embryo and endosperm of Arundinaria simonii f. heterophyllus was studied by means of paraffin section.And the accumulation dynamics of starch grains were observed by PAS reaction. The results were as follows: The zygote had a dormant for 5 days. The development of embryo belongs to graminad type . There are some stages during the development of embryo, including the stage of 2-cell proembryo, multicellular proembryo, globular proembryo, pyriform proembryo, differential embryo and mature embryo. The mature embryo is composed of radicle, coleorhiza, plumule, coleopt ile, scutellum and epiblast. The development of endosperm belongs to nuclear type. It includes three successive stages, which are free endosperm nucleus stage, cellularization stage and mature stage. Starch grains began accumulating around nuclear,16 days after pollination. Finally it filled the whole endosperm.

Key words: Arundinaria simonii f. heterophyllus, embryo, endosperm, PAS reaction

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