欢迎访问林业科学,今天是

林业科学 ›› 2009, Vol. 12 ›› Issue (4): 28-32.doi: 10.11707/j.1001-7488.20090405

• 论文 • 上一篇    下一篇

利用ACGM分子标记研究10个毛竹不同栽培变种的遗传多样性*

郭小勤 李犇 阮晓赛 娄永峰 林新春 汤定钦 方 伟   

  1. (浙江省现代森林培育技术重点实验室 临安 311300)
  • 收稿日期:2008-05-06 修回日期:1900-01-01 出版日期:2009-04-25 发布日期:2009-04-25
  • 通讯作者: 方伟

Genetic Diversity of Ten Phyllostachys edulis Cultivars with ACGM Markers

Guo Xiaoqin,Li Ben,RuanXiaosai,Lou Yongfeng,Lin Xinchun,Tang Dingqin,Fang Wei   

  1. (Key Laboratory for Modern Silvicultural Technology of Zhejiang Province Linan 311300)
  • Received:2008-05-06 Revised:1900-01-01 Online:2009-04-25 Published:2009-04-25

摘要:

应用40对ACGM引物扩增10个毛竹不同栽培变种及其2个近缘种材料,结果有35对引物可以在至少1个材料中得到特异性PCR产物,其中27对引物在12个供试材料中表现出多态性,占引物总数的67.5%。8对引物在供试材料间没有多态性位点,进一步对无多态性位点的扩增序列研究表明,相同引物扩增出的序列间差异性很小。从拷贝数来看,在水稻中表现为单拷贝的基因,有些在竹子中表现为多拷贝的特性;有些在部分供试材料中为单拷贝,而在另一些供试材料中表现为多拷贝。聚类结果表明: 在10个毛竹不同栽培变种中,绿槽毛竹和黄槽毛竹的亲缘关系最近; 圣音毛竹与其他不同栽培变种的毛竹亲缘关系最远。

关键词: ACGM分子标记, 毛竹, 遗传多样性

Abstract:

Genetic diversity of ten cultivars of Phyllostachys edulis and two affined species were investigated with 40 ACGM markers exploited from rice in this study. A total of 35 pairs of primers exhibited specific PCR products in at least one sample.Among those, 27 pairs of primers exhibited polymorphism PCR products in all samples, accounting for 67.5% of total primers. Eight pairs of primers had no polymorphism among all samples, and the sequences amplified with the same primer had little difference among randomly chosen five samples. Some genes, which were single copy in rice, exhibited multicopy in bamboo. The results of the cluster analysis showed that P. cv.Viridisulcata was closely related to P. cv. Luteosulcata, while P.cv. Tubaeformis had the fartherest relation to the other cultivars of P. edulis.

Key words: Phyllostachys edulis, genetic diversity