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林业科学 ›› 2019, Vol. 55 ›› Issue (4): 196-202.doi: 10.11707/j.1001-7488.20190421

• 研究简报 • 上一篇    

树脂铸型法研究毛竹材细胞壁的纹孔特征

刘嵘, 陈美玲, 刘贤淼, 费本华   

  1. 国际竹藤中心 北京 100102
  • 收稿日期:2017-02-27 修回日期:2017-07-21 出版日期:2019-04-25 发布日期:2019-04-30
  • 基金资助:
    国家自然科学基金项目“竹纤维细胞壁多层构造的理化表征”(31370563)。

Characteristics of Pits in Moso Bamboo(Phyllostachys edulis) Revealed by Resin Casting

Liu Rong, Chen Meiling, Liu Xianmiao, Fei Benhua   

  1. International Center for Bamboo and Rattan Beijing 100102
  • Received:2017-02-27 Revised:2017-07-21 Online:2019-04-25 Published:2019-04-30

摘要: [目的]研究毛竹材细胞壁纹孔的形态、结构和分布排列等特征,揭示维管束组织细胞间的纹孔通道,为竹子生长发育机制的深入研究和竹材高效利用提供理论依据。[方法]毛竹竹条经树脂渗透、树脂固化、组织消化和清洗干燥等处理后,获得单个细胞铸型和空间范围内多个细胞连接的立体铸型网络。结合毛竹切片扫描电镜图像,研究导管、薄壁细胞和纤维细胞的纹孔特征以及细胞间纹孔对的连接和分布特点。[结果]毛竹后生木质导管纹孔具明显的纹孔缘结构,并呈较高的选择性分布,即靠近维管束几何中心位置一侧的导管壁上往往密集分布大量纹孔,而另一侧纹孔则十分稀少或基本没有。导管铸型上,纹孔口和纹孔膜分别为0.9~2.7 μm和1.1~3.8 μm;维管束内存在小导管且相互间以具缘纹孔对连接;维管束内薄壁细胞纹孔比基本组织中的数量多、孔径大,紧邻木质部导管的薄壁细胞,其纹孔分布尤为密集;纤维细胞上纹孔稀疏分布,纹孔小、纹孔道狭长,纤维状管胞上分布有具缘纹孔;维管束内细胞间由纹孔对进行横向和纵向连接,并进一步形成流体移动的空间网络。[结论]树脂铸型法可直接展现纹孔立体结构,客观反映特定流体在竹材内部的扩散或渗透路径,适用于毛竹材细胞壁纹孔特征的研究。

关键词: 竹材, 细胞壁, 纹孔, 树脂铸型

Abstract: [Objective] To observe the pit morphology and distribution in moso bamboo, resin casting, a very proper way to obtain three-dimensional duplicate of pits, was applied.[Method] A two-component resin BS was used to complete the following procedures, infiltration of resin, resin curing, corrosion of bamboo tissue and dry cleaning in order. Resin casts of different types of individual cell and cells within certain scope served to reveal characteristics of pits in cell wall, pit pairs between cells and channels in vascular bundle. Moreover, a combination with observing bamboo sections was able to make verification of result getting from two ways.[Result] Pits in metaxylem vessels of moso bamboo presented a high selectivity of distribution, that is, large scale of pits were centered in single side of cell wall near to the geometric center of vascular bundle, though pits in the other side turned to be the opposite. And clear pit border structure was found. Diameter of pit aperture and pit membrane of vessel resin casts were 0.9-2.7 μm and 1.1-3.8 μm respectively. There were some smaller metaxylem vessel elements scattered or arranged adjacent connecting with bordered pit. As for parenchyma cells within the vascular bundle, pits were more and larger than those in the ground tissue. And parenchyma cells adjacent to metaxylem showed particularity. Pits in fiber cells were in sparse distribution with small openings, long and narrow cavities and suspect pit borders. Cells in vascular bundle connected each other through the pit and formatted space networks further.[Conclusion] Resin casting, which is capable of showing the pit stereo structure and reflecting diffusion permeation of specific fluid inside bamboo both directly and objectively, is a suitable method for investigating pits in moso bamboo.

Key words: bamboo, cell wall, pit, resin casting

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