应用微卫星分子标记估算毛竹的异交率

doi:10.11707/j.1001-7488.20140811

林业科学 ›› 2014, Vol. 50 ›› Issue (8): 76-81.doi: 10.11707/j.1001-7488.20140811

应用微卫星分子标记估算毛竹的异交率

刘政捷, 林源, 周明兵, 汤定钦

浙江农林大学亚热带森林培育省部共建国家重点实验室培育基地临安 311300

收稿日期:2013-10-16 修回日期:2014-06-05 出版日期:2014-08-25 发布日期:2014-07-31
基金资助:
“973”项目（2012CB723008）；浙江省重大农业专项（2010C12011，2012C12908-2）。

Estimation of Outcrossing Rate in Phyllostachys edulis by Microsatellite Polymorphism

Liu Zhengjie, Lin Yuan, Zhou Mingbing, Tang Dingqin

The Nurturing Station for the State Key Laboratory of Subtropical Silviculture Zhejiang A & F University Lin'an 311300

Received:2013-10-16 Revised:2014-06-05 Online:2014-08-25 Published:2014-07-31
Contact: 汤定钦

摘要/Abstract

摘要：

选用自行开发引物的、具有较高遗传多态性的8个微卫星位点，分析来自独立的3株开花毛竹半同胞家系各50个子代个体。结果表明：位于3个不同地点的开花植株都能够通过与外源花粉杂合产生具有不同的微卫星基因型特点的半同胞家系子代，各个家系子代内均存有一定的遗传多样性，估算的平均异交率约为0.089。这说明毛竹主要以自交为主，并兼顾有一定比例的异交以确保其后代种群的遗传多样性。

关键词: 毛竹, 微卫星, 遗传多样性, 交配系统, 异交率

Abstract:

Mating system is the basic property of plant populations and biological foundation for germplasm preservation as well as genetic improvement. In this study we applied developed 8 microsatellite loci with high polymorphism to analyze 50 half-sib progenies from each of 3 independent flowering culms of Phyllostachys edulis. The results showed that flowering culms in three different locations were fertilized with foreign pollen, which generated progenies with different microsatellite genotypes and genetic diversity. The overall estimates of outcrossing rate t_m of single-locus and t_s of multi-locus for the three culms were 0.089, indicating that the reproductive system of P. edulis predominantly involved in self-fertilization with a certain proportion of outcrossing to produce genetic diverse progenies. This is the first report so far on estimation of outcrossing rate for monopodial bamboo species such as P. edulis by DNA molecular markers.

Key words: Phyllostachys edulis, microsatellite, genetic diversity, mating system, rate of outcrossing

中图分类号:

S718.46

刘政捷, 林源, 周明兵, 汤定钦. 应用微卫星分子标记估算毛竹的异交率[J]. 林业科学, 2014, 50(8): 76-81.

Liu Zhengjie, Lin Yuan, Zhou Mingbing, Tang Dingqin. Estimation of Outcrossing Rate in Phyllostachys edulis by Microsatellite Polymorphism[J]. Scientia Silvae Sinicae, 2014, 50(8): 76-81.

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应用微卫星分子标记估算毛竹的异交率

Estimation of Outcrossing Rate in Phyllostachys edulis by Microsatellite Polymorphism

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