水杨酸诱导山茶抗灰斑病的作用及生理生化响应

doi:10.11707/j.1001-7488.20120215

林业科学 ›› 2012, Vol. 48 ›› Issue (2): 103-109.doi: 10.11707/j.1001-7488.20120215

水杨酸诱导山茶抗灰斑病的作用及生理生化响应

李姝江¹, 朱天辉¹, 黄艳娜², 韩珊¹, 谯天敏²

1. 四川农业大学林学院雅安 625014;2. 四川农业大学森林保护省级重点实验室雅安 625014

收稿日期:2011-04-07 修回日期:2011-12-28 出版日期:2012-02-25 发布日期:2012-02-25
通讯作者: 朱天辉

Roles of Salicylic Acid in Inducing Resistance of Camellia to the Gray Spot and the Biochemical Responses

Li Shujiang¹, Zhu Tianhui¹, Huang Yanna², Han Shan¹, Qiao Tianmin²

1. College of Forestry, Sichuan Agricultural University Ya'an 625014;2. Provincial Key Laboratory of Forest Protection, Sichuan Agricultural University Ya'an 625014

Received:2011-04-07 Revised:2011-12-28 Online:2012-02-25 Published:2012-02-25

摘要/Abstract

摘要：

采用载玻片萌发法测定水杨酸(SA)对山茶灰斑病菌的影响。浓度为0~5 mmol·L^-1的SA对山茶灰斑病菌的孢子萌发没有抑制作用,其诱发抗病性可持续10~15天,诱导最佳间隔期为3天。用SA喷雾涂布叶片诱导山茶抗性,植株内丙二醛、木质素、可溶性蛋白含量等生化指标对山茶灰斑病菌诱导信号有不同响应。MDA含量在一定时间范围内减少,膜脂的过氧化程度降低,但随着处理时间的延长,这种诱导抗性的作用逐渐减弱,且在不同浓度处理间存在差异,在诱导并挑战接种的处理中,各处理表现为初期低于对照、后期缓慢上升; 诱导并挑战接种的处理中,木质素含量的变化趋势与只诱导处理基本相似,高浓度诱导的木质素积累量大于低浓度; 诱导并挑战接种处理的可溶性蛋白含量与只诱导处理也表现出相同的变化规律,诱导并挑战接种处理的可溶性蛋白含量比只诱导的处理和对照高。生化反应与病情指数的相关性分析表明:MDA、木质素含量与病情指数达到显著水平(P<0.05)。

关键词: 诱导因子, 抗病性, 灰斑病, 七心红

Abstract:

The effects of salicylic acid (SA) on a pathogen (Pestalotiopsis guepinii) of Camellia were investigated by glass germination method. The result showed that the SA of 0~5 mmol·L^-1 did not inhibit growth of the pathogen. The induced resistance of camellia could last 10~15 days, and the optimum induced time was interval 3 days. The MDA, lignin, soluble protein had different responses to the disease resistance of camellia induced by spraying SA. MDA content in leaves decreased in certain period of time, suggesting that the membrane lipids peroxidation was decreased, but the effects of SA gradually were weakened with time. The responses were different with different SA concentrations. The experiment of induction and challenge inoculation showed that the MDA concentrations were lower than that in the control at initial stage, and then increased slowly at later stage. The treatment of induction and challenge inoculation brought about the same change trend of lignin content as the induction treatment. High SA concentrations were more effective than the low concentration. The contents of soluble proteins in the treatment of induction and challenge inoculation exhibited the same change trend as that in induction treatment, but were higher than that in the latter and the control. The correlation analysis of biochemical reaction and disease index showed there were significant (P<0.05) correlations between the contents of MDA, lignin and disease index.

Key words: induced factor, resistance, Pestalotiopsis guepinii, Camellia pitardii

中图分类号:

S763.1

李姝江;朱天辉;黄艳娜;韩珊;谯天敏. 水杨酸诱导山茶抗灰斑病的作用及生理生化响应[J]. 林业科学, 2012, 48(2): 103-109.

Li Shujiang;Zhu Tianhui;Huang Yanna;Han Shan;Qiao Tianmin. Roles of Salicylic Acid in Inducing Resistance of Camellia to the Gray Spot and the Biochemical Responses[J]. Scientia Silvae Sinicae, 2012, 48(2): 103-109.

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水杨酸诱导山茶抗灰斑病的作用及生理生化响应

Roles of Salicylic Acid in Inducing Resistance of Camellia to the Gray Spot and the Biochemical Responses

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