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林业科学 ›› 2011, Vol. 47 ›› Issue (6): 101-107.doi: 10.11707/j.1001-7488.20110615

• 论文 • 上一篇    下一篇

桉树抗青枯病的鉴定技术

王艳丽1, 李正楠2, 李会平1, 冉隆贤1   

  1. 1. 河北农业大学林学院 保定 071000;2. 河北农业大学河北省山区研究所 保定 071000
  • 收稿日期:2009-11-18 修回日期:2010-04-11 出版日期:2011-06-25 发布日期:2011-06-25
  • 通讯作者: 冉隆贤

Determination of Disease Resistance of Eucalypt against Bacterial Wilt Caused by Ralstonia solanacearum

Wang Yanli1, Li Zhengnan2, Li Huiping1, Ran Longxian1   

  1. 1. College of Forestry, Agricultural University of Hebei Baoding 071000;2. Mountain Area Research Institute of Hebei Province, Agricultural University of Hebei Baoding 071000
  • Received:2009-11-18 Revised:2010-04-11 Online:2011-06-25 Published:2011-06-25

摘要:

采用菌液浸泡法、顶尖接种法对不同的桉树无性系盆栽苗和生根组培苗进行抗青枯病性能测定,并结合林间调查筛选桉树抗青枯病树种和无性系,并从根系分泌物和组织研磨液的角度研究其对桉树青枯病菌生长的抑制作用,探索不同抗性桉树无性系抗病性强弱与其根系分泌物和组织研磨液之间的关系。结果表明: 供试的18个桉树无性系中,bd1、bd2、赤桉和南宁巨尾桉为抗病无性系; U6、南宁尾叶桉、雷9、钦32-29为中抗无性系; DH32-27、钦9、南宁广9、钦8、邓恩桉、钦32-22、雷2、巨桉、尾叶桉和钦广9为感病无性系。不同抗性桉树无性系根系分泌物和组织研磨液对青枯菌没有直接拮抗作用,但随着接种时间的延长,青枯病菌在抗病性强的无性系的根系分泌物及组织液中的增殖显著低于感病无性系,验证通过茎段浸泡接种筛选出的桉树无性系的准确性,同时表明根系分泌物和组织研磨液可以反映桉树不同无性系的抗病性强弱。

关键词: 桉树无性系, 青枯病, 抗病性, 根系分泌物, 组织研磨液

Abstract:

Resistance of Eucalyptus spp. against bacterial wilt, caused by Ralstonia solanacearum, was determined by dipping inoculation of shoot cuttings and shoot tip inoculation using potted seedlings and tissue culture plantlets, as well as field disease survey in a eucalypt plantation. The inhibition effects of root exudate and supernatant of tissue triturate collected from different resistant clones of Eucalyptus against R. solanacearum were performed using liquid culture to explore the relationship between exudate, tissue triturate and the levels of disease resistance. The results showed that the clones, bd1, bd2, E. camaldulensis and E. grandis×E. urophylla from Nanning were highly resistant to bacterial wilt; U6, E. urophylla from Nanning,Lei9 and Qin32-29 were the mid wilt-resistant clones; and DH32-27, Qin 9, Nanningguang 9, Qin8, E. dunnii, Qin32-22, Lei2, E. grandis, E. urophylla and Qinguang 9 were susceptible clones. The results also indicated that root exudates and the supernatant of triturated tissue had no direct inhibition to the growth of R. solanacearum. However, the proliferation number of R. solanacearum in root exudate and tissue triturate from wilt-susceptible clones was markedly higher than the ones from highly wilt-resistant clones, which was in accordance with the determination results of disease resistance using dipping inoculation and tip inoculation of shoot cuttings, indicating that the resistance ability of different clones could be manifested by the growth of R. solanacearum in root exudate and supernatant of triturated tissue.

Key words: clones of Eucalyptus, bacterial wilt, disease resistance, root exudate, tissue triturate

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