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林业科学 ›› 2010, Vol. 46 ›› Issue (4): 151-155.doi: 10.11707/j.1001-7488.20100423

• 研究简报 • 上一篇    下一篇

苏云金杆菌对松墨天牛幼虫的致死作用及其生物学特性

徐华潮1 徐金华1,2 张立钦1 林海萍1 杨萍3 程剑明2   

  1. 1.浙江林学院林业与生物技术学院 临安 311300; 2.浙江省淳安县林业局姜家林业中心站 淳安 311712;3.浙江省现代森林培育技术重点实验室 临安 311300
  • 收稿日期:2009-04-30 修回日期:1900-01-01 出版日期:2010-04-25 发布日期:2010-04-25

Virulence of Bacillus thuringiensis to Monochamus alternatus and Its biological Characteristics

Xu Huachao1,Xu Jinhua1,2,Zhang Liqin1,Lin Haiping1,Yang Ping3,Cheng Jianming2   

  1. 1.School of Forestry and Bio-Technology,Zhejiang Forestry University Linan 311300; 2. Jiangjia Forestry Central Station, Chun’an Forestry Bureau Chunan 311712;3. Key Laboratory for Modern Silvicultural Technology of Zhejiang Province Linan 311300
  • Received:2009-04-30 Revised:1900-01-01 Online:2010-04-25 Published:2010-04-25

关键词: 苏云金杆菌, 松墨天牛, 生物防治, 松材线虫病

Abstract:

The virulence of 17 Bacillus thuringiensis strains against 2-3 instar larvae of Monochamus alternatus were tested in indoor. The results showed that two strains (RBT-200701, RBT-200702) exhibited high poisonous activity, and the caused corrected mortality of 2-3 instar M. alternatus larvae were all more than 70%. A positive correlation was found between the corrected mortality and the concentration of the mixtures of spores and parasporal crystal (125-2 000 μg·g-1). The insecticidal effect increased with treatment time (0-20 d). The morphological changes of the two strains at various developmental stages were examined with microscopy on the cell smear. The optimal fermentation condition of strains was 30 ℃, pH7.2, 200 r·min-1, for 36 h. The two strains had the same protein spectrum after SDS-PAGE analysis.

Key words: Bacillus thuringiensis, Monochamus alternatus, biological control, pine wilt disease