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林业科学 ›› 2010, Vol. 46 ›› Issue (4): 146-150.doi: 10.11707/j.1001-7488.20100422

• 研究简报 • 上一篇    下一篇

盐胁迫下构树DREB转录因子基因表达的实时荧光定量PCR分析

杨帆1,2 丁菲2 杜天真2   

  1. 1. 湖州师范学院生命科学学院 湖州 313000; 2. 江西农业大学园林与艺术学院 南昌 330045
  • 收稿日期:2009-01-09 修回日期:1900-01-01 出版日期:2010-04-25 发布日期:2010-04-25
  • 通讯作者: 杜天真

DREB Gene Expression in Leaves of Broussonetia papyrifera Seedlings under Salt Stress Detected by Real-Time Fluorescent Quantitative PCR

Yang Fan1,2,Ding Fei2,Du Tianzhen2   

  1. 1.College of Life Sciences,Huzhou Teachers College Huzhou 313000; 2. College of Garden and Art, Jiangxi Agricultural University Nanchang 330045
  • Received:2009-01-09 Revised:1900-01-01 Online:2010-04-25 Published:2010-04-25

关键词: 实时荧光定量PCR, 构树, DREB转录因子, CT值, 盐胁迫

Abstract:

Real-time fluorescent quantitative PCR was used to datect DREB genes' expression levels in leaves of Broussonetia papyrifera seedlings at 0, 6, 12 and 24 h after treatments with 100, 200 and 300 mmol·L-1 NaCl. The results showed that BpDREB1 and BpDREB2 genes were induced by 100 and 200 mmol·L-1 NaCl to increase their expression levels. But the levels decreased at later stage by the salt stress. Treatment with 300 mmol·L-1 NaCl caused reduced expression levels of the two genes. The experiment verified that the transcriptional expression of DREB genes in B. papyrifera was able to be induced by salt stress, which might be involved in signalling pathways in response to the salt stress. But the genes were prohibited by higher salt concentration or longer duration of NaCl stress.

Key words: real-time fluorescent quantitative PCR, Broussonetia papyrifera, DREB transcription factor, CT value, salt stress