柳树种质资源遗传多样性和亲缘关系的CE-AFLP分析

doi:10.11707/j.1001-7488.20130606

林业科学 ›› 2013, Vol. 49 ›› Issue (6): 37-44.doi: 10.11707/j.1001-7488.20130606

柳树种质资源遗传多样性和亲缘关系的CE-AFLP分析

贾会霞, 吴立栓, 胡建军, 卢孟柱

林木遗传育种国家重点实验室国家林业局林木培育重点实验室中国林业科学研究院林业研究所北京 100091

收稿日期:2012-12-24 修回日期:2013-04-24 出版日期:2013-06-25 发布日期:2013-07-16
通讯作者: 胡建军
基金资助:
"863"计划(2011AA100201); 中央级公益性科研院所基本科研业务费(CAFYBB2012041)。

Genetic Diversity and Genetic Relationship of Salix Germplasms Revealed by CE-AFLP Analysis

Jia Huixia, Wu Lishuan, Hu Jianjun, Lu Mengzhu

State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of Forestry,CAF Beijing 100091

Received:2012-12-24 Revised:2013-04-24 Online:2013-06-25 Published:2013-07-16

摘要/Abstract

摘要：

利用毛细管电泳-AFLP技术,采用EcoRⅠ+3/MseⅠ+3和PstⅠ+2/MseⅠ+3引物组合,对从国内外搜集的37份柳树种质资源进行遗传多样性和亲缘关系分析。11对引物共扩增出1 580条条带,多态性条带数为1 399条,多态率在80.65%~94.67%之间,表明柳树种质间的遗传多样性较高。采用NTSYS-pc 2.10e软件对37份种质进行聚类分析和主成分分析,在相似系数0.63时,37份种质分为2大类,一类是乔木柳,一类除小乔木康定柳外,其余都为灌木柳。聚类分析和主成分分析结果与传统形态分类基本一致,但筐柳组中簸箕柳P295和沙柳基因组水平上差异较大,P295与银芽柳亲缘关系较近。研究结果为柳树分子生物学分类、种质资源保存与利用及品种改良等研究提供理论依据。

关键词: 柳树, 遗传多样性, 亲缘关系, CE-AFLP

Abstract:

Genetic diversity and genetic relationship of 37 Salix germplasms from domestic and foreign were analysed using the CE-AFLP(capillary electrophoresis-amplified fragment length polymorphism)technique. The primer combinations of EcoRI+3/MseI+3 and PstI+2/MseI+3 were used in this study. The result indicated that the 11 primer pairs were used to generate 1 580 fragments, of which 1 399 fragments were polymorphic. The polymorphism rate varied from 80.65% to 94.67%, showing a high genetic diversity among Salix. NTSYS-pc 2.10e software was used for cluster analysis and principal component analysis. The 37 germplasms were divided into two major categories with the similarity coefficient of 0.63, and one belonging to arbor Salix and others to the shrubs except for S. paraplesia. Cluster analysis and principal component analysis were generally consistent with traditional taxonomy. However, there was obvious difference in the genome level between S. suchowensis and S. psammophi in the section Helix. This study on the genetic diversity and genetic relationship of some Salix germplasms at the DNA level would provide a theoretical basis for Salix molecular classification, germplasm resources conservation and utilization, and variety improvement.

Key words: Salix, genetic diversity, genetic relationship, CE-AFLP

中图分类号:

贾会霞, 吴立栓, 胡建军, 卢孟柱. 柳树种质资源遗传多样性和亲缘关系的CE-AFLP分析[J]. 林业科学, 2013, 49(6): 37-44.

Jia Huixia, Wu Lishuan, Hu Jianjun, Lu Mengzhu. Genetic Diversity and Genetic Relationship of Salix Germplasms Revealed by CE-AFLP Analysis[J]. Scientia Silvae Sinicae, 2013, 49(6): 37-44.

参考文献

陈家辉, 孙航,杨永平.2008.柳树的分支系统学分析.云南植物研究,30(1): 1-7.

季鹏章,蒋会兵,黄兴奇,等.2009.古茶园、台地茶园遗传多样性的AFLP分析.遗传,31(1): 101-108.

李凤霞,王卫锋,王鲁,等.2010.烟草属植物遗传多样性和亲缘进化关系的荧光AFLP分析.中国农业科学,43(12): 2418-2427.

李洪,胡建军.2010.11个能源林杨柳无性系热值季节及年度变化.林业科学研究,23(3): 425-429.

吕金辉,胡建军,卢孟柱.2012.基于毛细管电泳的柳树AFLP分子标记研究.北京林业大学学报,34(1): 51-57.

王战,方振富.1984.中国植物志: 第20卷2分册.北京: 科学出版社.

朱继军,陈必胜,王玉勤,等.2012.苏柳、竹柳等速生能源柳引种研究.安徽农业科学,40(29): 14290-14291.

Allegro G, Giorcelli A, Deandrea G, et al. 2010. Damage assessment of the leafhopper Asymmetrasca decedens(Paoli, 1932)(Homoptera: Cicadellidae) on Salix spp. in Italy. Tercer Congreso Internacional de Salicáceas en Argentina,1(1): 1-6.

Allen G C, Flores-Vergara M A, Krasynanski S, et al. 2006. A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethyla-mmonium bromide. Nature Protocols, 1(5): 2320-2325.

Barker J H, Matthes M, Arnold G M, et al.1999. Characterisation of genetic diversity in potential biomass willows (Salix spp.) by RAPD and AFLP analyses. Genome, 42(2): 173-183.

Beismann H, Barker J, Karp A,et al.1997. AFLP analysis sheds light on distribution of two Salix species and their hybrid along a natural gradient. Molecular Ecology, 6(10): 989-993.

Brereton N J B, Pitre F E, Hanley S J, et al. 2010. QTL mapping of enzymatic saccharification in short rotation coppice willow and its independence from biomass yield. BioEnergy Research, 3(3): 251-261.

Huch G, Gauch J R. 1982. Multivariate analysis in community ecology. Cambridge University Press.

Karp A, Hanley S J, Trybush S O, et al. 2011. Genetic improvement of willow for bioenergy and biofuels free access. Journal of Integrative Plant Biology, 53(2): 151-165.

Kopp R, Smart L, Maynard C, et al. 2002. Predicting within-family variability in juvenile height growth of Salix based upon similarity among parental AFLP fingerprints. TAG Theoretical and Applied Genetics, 105(1): 106-112.

Luo M C, Thomas C, You F M, et al. 2003. High-throughput fingerprinting of bacterial artificial chromosomes using the snapshot labeling kit and sizing of restriction fragments by capillary electrophoresis. Genomics, 82(3): 378-389.

Pulford I, Watson C. 2003. Phytoremediation of heavy metal-contaminated land by trees. Environment International, 29(4): 529-540.

Trybush S, Jahodová Š, Macalpine W, et al. 2008. A genetic study of a Salix germplasm resource reveals new insights into relationships among subgenera, sections and species. BioEnergy Research, 1(1): 67-79.

Volk T, Abrahamson L, Nowak C, et al. 2006. The development of short-rotation willow in the northeastern United States for bioenergy and bioproducts, agroforestry and phytoremediation. Biomass and Bioenergy, 30(8/9): 715-727.

Vos P, Hogers R, Bleeker M, et al. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23(21): 4407-4414.

Wong K Y, Chuan Y C, Aggareal A, et al. 2004. Identifying patterns of DNA for tumor diagnosis using capillary electrophoresis-amplified fragment length polymorphism (CE-AFLP) screening. Journal of Bioinformatics and Computational Biology, 2(3): 569-587.

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柳树种质资源遗传多样性和亲缘关系的CE-AFLP分析

Genetic Diversity and Genetic Relationship of Salix Germplasms Revealed by CE-AFLP Analysis

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