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›› 2013, Vol. 49 ›› Issue (1): 61-67.doi: 10.11707/j.1001-7488.20130110

• 论文 • 上一篇    下一篇

泡桐属植物亲缘关系的ISSR分析

莫文娟1, 傅建敏1, 乔杰1, 雷莉莉1, 李芳东1, 袁德义2, 邱乾栋1   

  1. 1. 国家林业局泡桐研究开发中心 郑州 450003;2. 中南林业科技大学国家林业局经济林育种与栽培重点实验室 长沙 410004
  • 收稿日期:2012-03-13 修回日期:2012-06-08 出版日期:2013-01-25 发布日期:2013-01-25
  • 通讯作者: 李芳东

ISSR Study on Genetic Relationship of Genus Paulownia

Mo Wenjuan1, Fu Jianmin1, Qiao Jie1, Lei Lili1, Li Fangdong1, Yuan Deyi2, Qiu Qiandong1   

  1. 1. China Paulownia Research Center Zhengzhou 450003;2. Key Laboratory of Non-Wood Forest Product of State Forestry Administration Central South University of Forestry and Technology Changsha 410004
  • Received:2012-03-13 Revised:2012-06-08 Online:2013-01-25 Published:2013-01-25

摘要: 利用ISSR分子标记对泡桐属21份种、变种及变型材料进行亲缘关系分析。结果表明:从100个ISSR引物中筛选出9个扩增产物电泳后带型清晰可辨的引物,共扩增出85条DNA片段,其中多态性带74条,多态位点百分率为87.05%,反映出泡桐属不同种质间遗传差异明显,其丰富的多态性与供试材料广泛的种质代表性有关;UPGMA法将研究材料分成毛泡桐组、白花泡桐组和川泡桐组3大组,地理分布较近的种质聚类在一起,表现出较密切的亲缘关系;在对兰考泡桐、山明泡桐、白花兰考泡桐、楸叶泡桐、圆冠泡桐、亮叶毛泡桐、毛泡桐、白花泡桐、川泡桐的亲缘关系分析及分类处理上,基本上与形态学、细胞学、同工酶的研究结果一致。最后对泡桐属的部分植物的分类问题进行讨论。同时发现部分泡桐的一些特异性条带,可以进一步转化为SCAR标记。

关键词: 泡桐属, ISSR, 亲缘关系, 聚类分析

Abstract: In this study, 21 species, varieties and forma in Paulownia were used to detect the genetic relationships by using inter-simple sequence repeats(ISSR) markers. The results showed that: 1) A total of 85 DNA fragments were amplified using 9 out of 100 ISSR primers with unambiguous unique polymorphic bands, among which 74 DNA bands were polymorphic (PPB=87.05%), indicating significant genetic differentiation among Paulownia accessions tested; 2) The dendrogram was constructed with MEGA3.1 statistical package using UPGMA (the unweighted pair-group method with arithmetic average), with which Paulownia was roughly divided into three main groups of Paulownia tomentosa group, P. fortunei group and P. fargesii group. The Paulownia germplasms with close geographical distribution were clustered together, which illustrated there was a close relationship among them; 3) The findings of the relationship and classification were basically consistent with the morphology, cytology, isozyme study results among P. elongata, P. lamprophylla, P. elongata f. alba, P. catalpifolia, P. henanensis, P. tomentosa var. lucida, P. tomentosa, P. fortunei, and P. fargesii. At the end of this paper, the taxonomy of some Paulownia species was discussed. Some specific bands of Paulownia species, detected in this research, could be furthermore converted into SCAR marker.

Key words: Paulownia, ISSR, genetic relationship, cluster analysis

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