枣实蝇4个地理种群的线粒体Cytb基因序列分析

doi:10.11707/j.1001-7488.20140321

林业科学 ›› 2014, Vol. 50 ›› Issue (3): 144-150.doi: 10.11707/j.1001-7488.20140321

枣实蝇4个地理种群的线粒体Cytb基因序列分析

程晓甜¹, 阿地力·沙塔尔¹, 张伟², 李新泉¹

1. 新疆农业大学林学与园艺学院新疆教育厅干旱区林业生态与产业技术重点实验室乌鲁木齐 830052;
2. 新疆出入境检验检疫局技术中心乌鲁木齐 830083

收稿日期:2013-04-15 修回日期:2013-09-21 出版日期:2014-03-25 发布日期:2014-04-16
基金资助:
新疆维吾尔自治区科技计划项目“特色林果重大病虫持续高效绿色防控技术研究”（201130102-3）；新疆维吾尔自治区重点学科森林培育资助项目；新疆维吾尔自治区林业有害生物防治检疫局项目“枣实蝇快速检测和监测技术研发”。

Sequence Analysis of Mitochondrial Cytb Gene of Four Geographic Populations of Carpomya vesuviana

Cheng Xiaotian¹, Adil Sattar¹, Zhang Wei², Li Xinquan¹

1. Key Laboratory of Forestry Ecology and Industry Technology in Arid Region, Education Department of Xinjiang College of Forestry and Horticulture, Xinjiang Agricultural University Urumqi 830052;
2. Xinjiang Entry-Exit Inspection and Quarantine Bureau Urumqi 830083

Received:2013-04-15 Revised:2013-09-21 Online:2014-03-25 Published:2014-04-16
Contact: 阿地力·沙塔尔

摘要/Abstract

关键词: 枣实蝇, Cytb, 进化树, 亲缘关系

Abstract:

In this study, PCR amplification and sequencing techniques were used to compare and analyze mitochondrial Cytb gene sequences of four geographic populations (KSS, Turpan, HSX and HMS) of Carpomya vesuviana, to explore the genetic relationship among them. 469 bp fragments were obtained.The composition,specific site and base transition/transversion frequency of the partial sequences of Cytb gene were analyzed. The frequencies of codon usage were calculated and the phylogenetic tree was reconstructed. The results showed that KSS population has a relatively far genetic relationship with Turpan population, HSX population and HMS population.

Key words: Carpomya vesuviana, Cytb, phylogenetic tree, genetic relationship

中图分类号:

Q965

程晓甜, 阿地力·沙塔尔, 张伟, 李新泉. 枣实蝇4个地理种群的线粒体Cytb基因序列分析[J]. 林业科学, 2014, 50(3): 144-150.

Cheng Xiaotian, Adil Sattar, Zhang Wei, Li Xinquan. Sequence Analysis of Mitochondrial Cytb Gene of Four Geographic Populations of Carpomya vesuviana[J]. Scientia Silvae Sinicae, 2014, 50(3): 144-150.

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枣实蝇4个地理种群的线粒体Cytb基因序列分析

Sequence Analysis of Mitochondrial Cytb Gene of Four Geographic Populations of Carpomya vesuviana

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