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林业科学 ›› 2005, Vol. 41 ›› Issue (5): 51-55.doi: 10.11707/j.1001-7488.20050509

• 论文及研究报告 • 上一篇    下一篇

观赏竹的试管快繁研究

王光萍 丁雨龙 黄敏仁 王明庥   

  1. 南京林业大学,南京210037
  • 收稿日期:2004-07-26 修回日期:1900-01-01 出版日期:2005-09-25 发布日期:2005-09-25

Tissue Culture of Some Ornamental Bamboos

Wang Guangping,Ding Yulong,Huang Minren,Wang Mingxiu   

  1. Nanjing Forestry University Nanjing 210037
  • Received:2004-07-26 Revised:1900-01-01 Online:2005-09-25 Published:2005-09-25

摘要:

对菲白竹、平安竹、金镶玉竹、锦竹、菲黄竹、花秆佛肚竹、秋竹、爬竹、香竹、刺黑竹和水竹等11种观赏竹种,分别采用成年竹当年新萌发嫩芽或当年生带侧芽枝条为初始外植体,以MS为基本培养基,附加不同种类和浓度的植物生长调节物质诱导丛生芽及再生植株。结果表明:外植体经诱导产生丛生芽后,亲缘关系较近的竹种,丛生芽增殖培养基有一定相似性;适宜的生根培养基是试管快繁成功的关键,不同竹种丛生芽生根难易差别较大,丛生竹生根率可达95%以上,而散生竹则较难生根,加入一定浓度的细胞分裂素BA,生根率可达65%~90%不等。再生植株在自动间歇式喷雾下的纯蛭石基质中移栽成活率可达90%左右。通过对再生植株表型相关性状观察,多数种类未发生明显的变异。采用已开花植株新萌发的嫩芽进行组织培养,再生植株易出现开花现象。

关键词: 观赏竹, 组织培养, 快速繁殖, 表型变异

Abstract:

The lateral young buds from branches of 11 bamboos, like Pleioblastus fortunei, Pl. viridistriatus, Pseudosasa japonica cv. Tsutsumiana, Phyllostachys aureosulcata f. speciosa, Ph. Heteroclada, Hibanobambusa tranquillans, Chusquea culeou,Chimonocalamus delicatus,Drepanostachyum scandens, Chimonobambusa neopurpurea and Bambusa ventricosa cv. Kimmei, were used as explants. The clustered adventitious buds were induced in 20~60 days after inoculation for different species respectively. Subcultures were performed at intervals of 20~40 days by separation of the multiple shoots in groups of three to five and transferred to fresh medium. The rooting rate for monopodial bamboos was 65%~90%, while for the sympodial ones more than 95%. The rooting rate was of great difference among species. The survival rate of the plantlets was about 90%. According to the observation, the most of plantlet from tissue culture can keep the original morphological characters.

Key words: ornamental bamboos, tissue culture, rapid propagation, morphological variation