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林业科学 ›› 2009, Vol. 12 ›› Issue (8): 34-38.doi: 10.11707/j.1001-7488.20090806

• 论文 • 上一篇    下一篇

杂种落叶松未成熟胚的体细胞胚发生和植株再生

王伟达1 李成浩1 杨静莉1 张含国1 张淑玲2   

  1. 1.东北林业大学 林木遗传育种与生物技术教育部重点实验室哈尔滨 150040; 2.抚顺矿业集团有限责任公司林业处抚顺 113008
  • 收稿日期:2008-05-14 修回日期:1900-01-01 出版日期:2009-08-25 发布日期:2009-08-25
  • 通讯作者: 李成浩
  • 基金资助:
     

Somatic Embryogenesis and Plantlet Regeneration from Immature Zygotic Embryos of Hybrid Larch

Wang Weida1,Li Chenghao1,Yang Jingli1,Zhang Hanguo1,Zhang Shuling2   

  1. 1.Key Laboratory of Forest Genetic Improvement and Biotechnology of Ministry of EducationNortheast Forestry UniversityHarbin 150040;2.Forestry Department, Fushun Mining Group Limited CompanyFushun 113008
  • Received:2008-05-14 Revised:1900-01-01 Online:2009-08-25 Published:2009-08-25
  • Supported by:
     

摘要:

以未成熟合子胚为外植体,建立杂种落叶松胚性愈伤组织诱导和植株再生体系。从日本落叶松5×长白落叶松77-3和日本落叶松5×兴安落叶松9两个家系诱导出胚性愈伤组织,授粉后65天左右采集的合子胚在添加0.5 mg·L-1 2,4-D + 0.5 mg·L-1 6-BA + 0.5 mg·L-1 KT的S培养基上胚性愈伤组织诱导率最高,为10%。从胚性愈伤组织中筛选出3个具有稳定分化能力的胚性细胞系,其中授粉后65天采集的日本落叶松5×长白落叶松77-3合子胚诱导的胚性细胞系的每克胚性愈伤组织形成的体胚数、体胚萌发率、植株再生率最高,分别为18.1个,77.0%,28.1%。再生植株的移栽成活率为41.8%。

关键词: 杂种落叶松, 胚性愈伤组织, 合子胚, 组织培养

Abstract:

This paper reported that an in vitro embryogenic callus induction and plantlet regeneration system from immature zygotic embryos of hybrid larch was established. Embryogenic calli were induced from zygotic embryos of Larix kaempferi 5×L. olgensis 77-3 and Larix kaempferi 5×L. gmelinii 9. The embryogenic callus induction rate was the highest when zygotic embryos were collected 65-days after pollination and cultured on S medium supplemented with 0.5 mg·L-1 2,4-D + 0.5 mg·L-1 6-BA + 0.5 mg·L-1 KT, with 10% embryogenic callus induction rate. Three embryogenic cell lines were found to have stable regenerating ability. The cell lines from zygotic embryos of L.kaempferi 5×L.olgensis 77-3 collected 65-days after pollination were the best, with 18.1,77.0%, and 28.1% of number of somatic embryo per gram embryogenic callus, germination rate and plant regeneration rate, respectively. Up to 41.8% of the transplanted plantlets were successfully survived in soil.

Key words: hybrid larch, embryogenic callus, zygotic embryo, tissue culture

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