林业科学 ›› 2011, Vol. 47 ›› Issue (5): 157-161.doi: 10.11707/j.1001-7488.20110526
• 研究简报 • 上一篇 下一篇
燕丽萍, 夏阳, 毛秀红, 刘翠兰, 王太明, 李丽, 李双云
收稿日期:
修回日期:
出版日期:
发布日期:
通讯作者:
Yan Liping, Xia Yang, Mao Xiuhong, Liu Cuilan, Wang Taiming, Li Li, Li Shuangyun
Received:
Revised:
Online:
Published:
关键词: 邓恩桉, 组织培养, 微繁殖
Abstract:
It is very difficult for Eucalyptus dunnii to produce roots in vitro. In order to resolve this problem, produce E. dunnii seedlings, and conduct gene engineering, an efficient and rapid micropropagation system by using seeds as explants was established for E. dunnii.The optimum medium for E. dunnii was obtained by modifying Mecown and Lloyd medium (WPM), that is: with WPM large elements, plus Murashige and Skoog (MS) trace elements, MS Fe salt, Skirvin and Chu (SC) organic compounds and 30 g ·L-1 glucose. This modified medium was used to resolve the serious problems, such as seedling brown and shoot tip death, in E. dunnii tissue culture. A modified WPM medium supplemented with 0.5 mg ·L-1 6-benzy1aminopurine, 0.5 mg ·L-1 kinetin, 0.2 mg ·L-1 napthaleneacetic acid and 30 g ·L-1 glucose was suitable for shoot induction and proliferation from divided buds,and the shoot proliferation rate of plantlets increased by 18.43 fold. The optimum growing medium was obtained by modifying WPM medium supplemented with 0.5 mg ·L-1 KT, 0.2 mg ·L-1 TDZ and 0.01 mg ·L-1 NAA. Over 84.3% regeneration plantlets were able to root after transferred to the modified half-strength MS medium supplemented with 3.0 mg ·L-1 indole-3-butyric acid, 0.01 mg ·L-1 napthaleneacetic acid, 0.05 mg ·L-1 kinetin and 20 g ·L-1 sucrose. When the micropropagated plantlets with well-developed root systems were transferred to planting bed containing a mixture of sand,soil and medium (4 ∶1 ∶1;V/V) in a greenhouse,93.8% of the plantlets survived.
Key words: Eucalyptus dunnii, tissue culture, micropropagation
中图分类号:
S723.1+3
Q943.1
燕丽萍;夏阳;毛秀红;刘翠兰;王太明;李丽;李双云. 邓恩桉的组织培养[J]. 林业科学, 2011, 47(5): 157-161.
Yan Liping;Xia Yang;Mao Xiuhong;Liu Cuilan;Wang Taiming;Li Li;Li Shuangyun. Tissue Culture of Eucalyptus dunnii[J]. Scientia Silvae Sinicae, 2011, 47(5): 157-161.
0 / / 推荐
导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks
链接本文: http://www.linyekexue.net/CN/10.11707/j.1001-7488.20110526
http://www.linyekexue.net/CN/Y2011/V47/I5/157