林业科学 ›› 2004, Vol. 40 ›› Issue (4): 189-192.doi: 10.11707/j.1001-7488.20040434
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韩秀慧 尹伟伦 王华芳
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Han Xiuhui,Yin Weilun,Wang Huafang
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关键词: 二次回归正交设计, 微型月季, 组织培养, 6-BA, NAA
Abstract:
Quadratic regressive factorial experiment was used to optimize concentrations of 6-BA and NAA added in MS midum for shoot multiplication of miniature rose (Rosa cv.Sonnen Kind).The most amount of shoots was achieved by combining 6-BA 1.93 mg·L-1, NAA 0.33 mg·L-1into MS midum. The 6-BA and NAA concentrations were in a range of 1.68～2.75 mg·L-1 and 0.07～0.54 mg·L-1, respectively, and each 10 explants (stems with one auxiliary bud) cultured on the midum for 40 days, then 50 shoots occurred. The probability of the event was 95%.
Key words: Quadratic regression factorial experiment, Miniature rose, in vitro culture, 6_BA, NAA
韩秀慧 尹伟伦 王华芳. 二次回归正交设计在微型月季组织培养中的应用[J]. 林业科学, 2004, 40(4): 189-192.
Han Xiuhui;Yin Weilun;Wang Huafang. Application of Quadratic Regressive Factorial Experiment to in vitro Culture of Miniature Rose[J]. Scientia Silvae Sinicae, 2004, 40(4): 189-192.
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