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林业科学 ›› 2008, Vol. 44 ›› Issue (6): 168-170.doi: 10.11707/j.1001-7488.20080628

• 论文 • 上一篇    下一篇

桑树组织培养诱导多倍体植株 *

王茜龄1 周金星2 余茂德1 徐 立1 李镇刚1   

  1. (1.西南大学生物技术学院 北碚400716;2.中国林业科学研究院林业研究所 国家林业局林木培育重点实验室 北京100091)
  • 收稿日期:2007-05-25 修回日期:1900-01-01 出版日期:2008-06-25 发布日期:2008-06-25
  • 通讯作者: 余茂德

Induction of Polyploids on Mulberry in Tissue Culture

Wang Xiling1,Zhou Jinxing2,Yu Maode1,Xu Li1,Li Zhengang 1

  

  1. (1.College of Biotechnology,Southwest University Beibei 400716;2. Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of Forestry,CAF Beijing 100091)
  • Received:2007-05-25 Revised:1900-01-01 Online:2008-06-25 Published:2008-06-25

关键词: 桑树, 下胚轴, 组织培养, 秋水仙碱, 多倍体植株

Abstract:

Under aseptic conditions, hypocotyls of mulberry were averagely cut into 3 segments which were then cultured on MS solid medium supplemented with 30 mg\5L-16BA, 0.3 mg\5L-1IAA, 1.0 mg\5L-1AgNO3 to select the best region of hypocotyls for getting the more caesptose shoots. In this study, the tip part of hypocotyls(near the germ) developed quickly and induced adventitious buds easily.The caesptose shoots were dipped into 1,1.5, 2.5 g\5L-1 colchicines solution for 2 or 3 days, or dropped with 1.5, 2, 2.5 g\5L-1 colchicines solution 2 times a day for 5 days, and then transferred to fresh medium. Both the dipped and dropped treatments induced variant shoots. Examination of the chromosomes of the shoot tip cells of the regenerated plantlets revealed that they were either tetraploids or chineras. The rate of tetraploids produced from the dipped and dropped treatments was 14% and 20% respectively with the chromosomes number of 56(2n=4x=56) as compared to the diploid(2n=2x=28).

Key words: mulberry(Morus), hypocotyls, tissue culture, colchicines, polyploidy