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林业科学 ›› 2008, Vol. 44 ›› Issue (12): 57-61.doi: 10.11707/j.1001-7488.20081210

• 论文 • 上一篇    下一篇

平榛组织培养与快速繁殖

程云清 刘剑锋 陈智文   

  1. 吉林师范大学,四平136000
  • 收稿日期:2008-01-16 修回日期:1900-01-01 出版日期:2008-12-25 发布日期:2008-12-25
  • 通讯作者: 陈智文

Tissue Culture and Rapid Propagation of Corylus heterophylla

Cheng Yunqing,Liu Jianfeng,Chen Zhiwen   

  1. Jilin Normal University Siping 136000
  • Received:2008-01-16 Revised:1900-01-01 Online:2008-12-25 Published:2008-12-25

摘要: 以平榛茎段为外植体,进行不定芽的诱导和快速繁殖研究。结果表明:初代培养基DKW+5mg·L-16-BA+0.01mg·L-1IBA中加入维生素C2.0g·L-1可有效抑制褐化的发生。附加64.42mg·L-1腐胺、58.10mg·L-1亚精胺和20.20mg·L-1精胺有利于外植体芽体的萌发与生长,经初代培养后茎段切口可产生愈伤组织,并可直接成芽;以产生的愈伤组织为材料,在培养基DKW+0.01mg·L-1IBA+2.5mg·L-1TDZ+32.21mg·L-1腐胺+29.05mg·L-1亚精胺+10.10mg·L-1精胺上,平榛愈伤组织可诱导分化出不定芽,加多胺的处理有利于提高芽体萌发率和加快生长。在培养基1/2MS+IBA0.25mg·L-1上生根率达90%以上。

关键词: 平榛, 组织培养, 快速繁殖

Abstract: Bud induction and fast propagation were carried out by using shoot segments containing auxiliary buds of Corylus heterophylla as primary materials. The results showed that, primary medium DKW+5 mg·L-1 6-BA+0.01 mg·L-1 IBA supplemented with 2.0 g·L-1 vitamin C was profitable to prevent browning of explants, and addition of 64.42 mg·L-1 putrescine,58.10 mg·L-1 spermidine and 20.20 mg·L-1 spermine promoted bud germination rate and growth speed. Calli or buds occurred from the cuts of shoot segments on the primary medium. The calli were transferred to medium DKW+0.01 mg·L-1 IBA+2.5 mg·L-1 TDZ+32.21 mg·L-1 putrescine+29.05 mg·L-1 spermidine+ 10.10 mg·L-1 spermine and induced to produce adventitious buds. The treatments with polyamines were profitable to promote buds germination and growth. Over 90% regeneration plantlets were able to root after transferred to medium 1/2MS + IBA 0.25 mg·L-1 .

Key words: Corylus heterophylla, tissue culture, rapid propagation