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林业科学 ›› 2002, Vol. 38 ›› Issue (6): 125-136.doi: 10.11707/j.1001-7488.20020621

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植原体分子分类的现状与问题

秦国夫 赵俊 刘小勇   

  1. 国家林业局森林病虫害防治总站,沈阳110034;中国科学院微生物研究所,北京100080
  • 收稿日期:2001-05-15 修回日期:1900-01-01 出版日期:2002-11-25 发布日期:2002-11-25

CURRENT STATUS AND PROBLEMS OF MOLECULAR CLASSIFICATION IN THE PHYTOPLASMAS

Qin Guofu,Zhao Jun,Liu Xiaoyong   

  1. General Station of Forest Pest Control,SAF Shenyang110034;Institute of Microbiology,Academia Sinica Beijing100080
  • Received:2001-05-15 Revised:1900-01-01 Online:2002-11-25 Published:2002-11-25

摘要:

从20世纪80年代末开始,植原体的DNA分子分类研究取得了实质性进展,植原体的系统学位置被确定为柔膜菌纲的一个单系发育类群,作为一个暂定属(Candidatus genus)处理。利用16SrDNA、16S/23S间隔区、rp、Tuf等一系列基因序列及其RFLP图谱,对全世界近300种植原体进行了分子分类。按照李英敏和Seemuller的分类系统,植原体分别被划分为14个16SrDNA类群和20个16SrDNA类群。根据ICSB的规定,每一个16SrDNA类群作为一个暂定种(Candidatus species)。每个16SrDNA类群内有较大的遗传分化,可分为很多亚组。目前全世界共发表了5个植原体暂定种,分别为“Candidatus Phytoplasma aurantifolia”,“Candidatus P.australiense”,“Candidatus P.australasia”,“Candidatus P.fraxini”和“Candidatus P.japonicum”。其中的4个种是16SrDNA类群的亚组。植原体暂定种的概念还存在一些理论和实践上的问题。文中列出了李英敏的分类框架,并讨论了植原体分子分类的发展方向。

关键词: 植原体, 暂定种, 分子分类

Abstract:

The molecular taxonomy of Phytoplasmas achieved the tremendous progress due to DNA-based method was introduced from 1990s,the Phytoplasma was determined as a candidatus genus of the Mullicutes.At present,through combining DNA molecular data such as RFLP patterns,DNA sequences,nearly three hundred Phytoplasmas were classified into 14 or 20 major phylogenetic groups according to the Lee or Semmuller identification scheme,respectively.Every group or subgroup was representative of candidatus species of Phytoplasma,totally five candidatus species of Phytoplasma were published,i.e.“Candidatus Phytoplasma aurantifolia”,“Candidatus P.australiense”,“Candidatus P.australasia”,“Candidatus P.fraxini” and “Candidatus P.japonicum”.The Lee's identification scheme was listed.The problems of candidatus species concept in Phytoplasmas were discussed in detail.

Key words: Phytoplasmas, Candidatus species, Molecular taxonomy