Scientia Silvae Sinicae ›› 2024, Vol. 60 ›› Issue (3): 10-21.doi: 10.11707/j.1001-7488.LYKX20230129
• Frontier & focus: Cultivation physiology and fruit quality of Lycium barbarum • Previous Articles Next Articles
Xuerui Feng1,Yaping Ma2,Jiaxin Liu2,Hui Lu2,Yunmao Li1,Bing Cao2,*
Received:2023-04-02
Online:2024-03-25
Published:2024-04-08
Contact:
Bing Cao
CLC Number:
Xuerui Feng,Yaping Ma,Jiaxin Liu,Hui Lu,Yunmao Li,Bing Cao. Analysis of Enzyme and Gene Expression Related to Sugar Metabolism in Lycium barbarum under Elevated CO2 Concentration Treatment[J]. Scientia Silvae Sinicae, 2024, 60(3): 10-21.
Fig.1
Four fruit development stages and morphological characteristics of root, stem and leaf of L.bararum cv. ‘Ningqi-1’"
Table 1
Sequence information of primers used in fluorescence quantitative PCR"
| 序列号 Accession No. | 基因名称 Gene name | 引物方向 Primer direction | 引物序列Primer sequence(5'–3') |
| Actin | 正向 Forward | CCATCTACGAGGGTTACGCTTTG | |
| 反向 Reverse | AGTCAAGAGCCACATAGGCAAGC | ||
| MH025913.1 | LbGALA | 正向 Forward | TGCTGCTATGGTGCTGCTTGTG |
| 反向 Reverse | GCGTTGGCTGTGGAGTTTGAATC | ||
| MH025912.1 | LbMS | 正向 Forward | TGAGGCAGCATTGGAACTTGTAAGG |
| 反向 Reverse | AGGTGCATTGGTCATGTTACTGGTG | ||
| MH025911.1 | LbGAE | 正向 Forward | GCCGATTTCACTGTTTCAAGGTTCC |
| 反向 Reverse | TCCTGTGCTCTTTTCTGCTGTATCC | ||
| MN718198 | LbNI | 正向 Forward | TGGTGAGAGTGCGATAGGAAGAGTG |
| 反向 Reverse | GGCATTCAGGCATCTCAGACAAGG | ||
| MN718197 | LbSPS | 正向 Forward | TGAAGGGTGCTGTCGAGGAA |
| 反向 Reverse | TTCCCGCTGGTGTAGGTGAT | ||
| MN718196 | LbSS | 正向 Forward | ACCATTTCTCAGCCCAGTTTACGG |
| 反向 Reverse | GTCCAACGGTGTCCTTGCTTCC | ||
| MN718195 | LbAI | 正向 Forward | AGCCGCACAGTTGGATATAGAAGC |
| 反向 Reverse | CTCCACTAGAACCGCAGTTGTAACC |
Fig.2
Effects of elevated CO2 concentration on the content of sugar components in fruits of L.bararum cv. ‘Ningqi-1’ at different developmental stages Different small letters a, b, c, d indicated that there was a significant difference in the development period of the same treatment at the 0.05 level; * indicates that the difference between different treatments in the same period is significant at the 0.05 level; * * represents a significant difference at 0.01 level. YF: young fruit period, GF: green fruit period, CF: color-changing period, RF: red fruit period, CK: natural environment CO2 concentration treatment, TR: elevated CO2 concentration treatment."
Fig.3
Effects of elevated CO2 concentration on the activities of sucrose metabolism-related enzymes in L.bararum cv.‘Ningqi-1’ In each subplot, the nine small plots present: (Upper-left) the variations of enzymatic activities with respect to organizations in different treatments; (Upper-middle) the variations of enzymatic activities with respect to stages in different treatments; (Upper-right) the box-plot of enzymatic activities at each treatments; (Middle-left) the variations of enzymatic activities with respect to organizations in different stages; (Middle) the box-plot of enzymatic activities at each stages; (Middle-right) the variations of enzymatic activities with respect to treatments in different stages; (Lower-left) the box-plot of enzymatic activities at each organizations; (Lower-middle) the variations of enzymatic activities with respect to stages in different organizations; (Lower-right ) the variations of enzymatic activities with respect to treatments in different organizations. Different small letters a，b，c，d indicated that there was a significant difference in the development period of the same treatment at the 0.05 level. YF: young fruit period, GF: green fruit period, CF: color-changing period, RF: red fruit period, CK: natural environment CO2 concentration treatment, TR: elevated CO2 concentration treatment ."
Fig.4
Effects of elevated CO2 concentration on the activities of three galactose metabolism-related enzymes in L.bararum cv.‘Ningq-1’ In each subplot, the nine small plots present: (Upper-left) the variations of enzymatic activities with respect to organizations in different treatments; (Upper-middle) the variations of enzymatic activities with respect to stages in different treatments; (Upper-right) the box-plot of enzymatic activities at each treatments; (Middle-left) the variations of enzymatic activities with respect to organizations in different stages; (Middle) the box-plot of enzymatic activities at each stages; (Middle-right) the variations of enzymatic activities with respect to treatments in different stages; (Lower-left) the box-plot of enzymatic activities at each organizations; (Lower-middle) the variations of enzymatic activities with respect to stages in different organizations; (Lower-right ) the variations of enzymatic activities with respect to treatments in different organizations. Different small letters a，b，c，d indicated that there was a significant difference in the development period of the same treatment at the 0.05 level. YF: young fruit period, GF: green fruit period, CF: color-changing period, RF: red fruit period, CK: natural environment CO2 concentration treatment, TR: elevated CO2 concentration treatment."
Fig.5
The expression pattern of glucose metabolism related genes in L. bararum cv.‘Ningqi-1’:under elevated CO2 concentration (a) The up-regulated and down-regulated expression of glucose metabolism genes in different tissues at four developmental stages, YF: young fruit period, GF: green fruit period, CF: color-changing period, RF: red fruit period. (b) The relative expression levels of LbAI, LbSS, LbSPS, LbNI, LbGAE, LbGALA and LbMS genes in different tissues at four developmental stages under elevated atmospheric and CO2 concentrations. ** P<0.01, * P<0.05."
Fig.6
Correlation analysis of elevated CO2 concentration on sugar, sugar metabolism enzymes and their genes in L.bararum cv.‘Ningqi-1’ fruit The box color represents the positive or negative correlation coefficient, and the size represents the degree of correlation, The width of the connection（Mantel’s r） represents the correlation of the mantel test, and the color of the line（Mantel’s P） represents the statistical significance, ** P<0.01，* P<0.05. YF: young fruit period, GF: green fruit period, CF: color-changing period, RF: red fruit period, Mantel test is a correlation analysis of the two matrices, as shown in Fig.a, YF, GF, CF, RF four points represent the expression levels of seven sugar metabolism-related genes in young fruit stage, green fruit stage, veraison stage and red fruit stage, respectively; in Fig.b, the four points of root, stem, leaf and fruit on the left side represent seven enzyme activities related to sugar metabolism in root, stem, leaf and fruit, respectivel."
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