荧光假单胞菌与红绒盖牛肝菌共接种对杨树根际土壤酶活性及微生物多样性的影响

doi:10.11707/j.1001-7488.20190103

Abstract

Abstract: [Objective] Under co-inoculation with Pseudomonas fluorescens JW-JS1 and Xerocomus chrysenteron, the changes of enzyme activity and the microbial functional diversity in rhizosphere soil of NL-895 Poplar were studied to reveal the interaction mechanism by co-inoculation with phosphate-solubilizing bacteria and ectomycorrhizal fungi at the micro-ecological level.[Method] A pot experiment was conducted to investigate the effects of co-inoculation with Pseudomonas fluorescens JW-JS1 and Xerocomus chrysenteron(Xc) on the activities of soil enzymes (acid phosphatase, dehydrogenase and invertase), and the BIOLOG microplate method was applied to assay the microbial community and its function diversity in rhizosphere soil of NL-895 Poplar.[Result] The results showed that the soil enzymes activities (acid phosphatase, dehydrogenase and invertase) in rhizosphere of NL-895 Poplar with different inoculation were all higher than those of control, especially for acid phosphatase and invertase when co-inoculation with JW-JS1 and Xc. The BIOLOG plates analysis showed that AWCD values on day 150 after inoculation were generally higher than those of control (except for 24 hour and 48 hour), and the value first increased then stabilized with the prolongation of incubation time. After 96 hour incubation, the McIntosh index and Richness index of co-inoculation with JW-JS1+Xc were higher than single inoculation with Xc or JW-JS1 and those of control (P<0.05), but the Shannon-Wiener index and Simpson index were significantly lower (P<0.05). There was significant difference in the utilization ability of six carbon sources in BIOLOG microplates by soil microorganism in rhizosphere of NL-895 Poplar with different inoculation treatments (P<0.05).[Conclusion] The co-inoculation of Pseudomonas fluorescens JW-JS1 and Xerocomus chrysenteron may play roles in improving the activities of soil enzymes and enriching soil microbial communities. All of these are conductive to improve soil fertility and promote the growth of NL-895 poplar. The results also showed that co-inoculation with PSB + ECF could effectively increase the activity of soil enzymes and improve soil biodiversity compared with single inoculation with PSB or ECF, which could provide a theoretical basis for developing the compound microbial fertilizer in the future.

Key words: Pseudomonas fluorescens, Xerocomus chrysenteron, co-inoculation, soil enzyme activity, BIOLOG microplate, diversity of microbial community

CLC Number:

S718.7

Liu Hui, Wu Xiaoqin, Ren Jiahong, Chen Dan. Effect of Co-Inoculation with Pseudomonas fluorescens and Xerocomus chrysenteron on the Soil Enzyme Activity and Microbial Diversity in Poplar Rhizosphere[J]. Scientia Silvae Sinicae, 2019, 55(1): 22-30.

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Effect of Co-Inoculation with Pseudomonas fluorescens and Xerocomus chrysenteron on the Soil Enzyme Activity and Microbial Diversity in Poplar Rhizosphere

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