Scientia Silvae Sinicae ›› 2021, Vol. 57 ›› Issue (9): 110-120.doi: 10.11707/j.1001-7488.20210911
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Xingzhou Chen,Guoying Zhou,Xinggang Chen,Lingyu Jiang,Anhua Bao,Jun Liu*
Received:
2020-09-01
Online:
2021-09-25
Published:
2021-11-29
Contact:
Jun Liu
CLC Number:
Xingzhou Chen,Guoying Zhou,Xinggang Chen,Lingyu Jiang,Anhua Bao,Jun Liu. Screening of Effectors of Colletotrichum fructicola in Camellia oleifera[J]. Scientia Silvae Sinicae, 2021, 57(9): 110-120.
Table 1
9 candidate effectors gene-specific primer sequences designed for qRT-PCR"
基因ID GenBank ID | 扩增长度Amplification length/bp | 正向引物Forward primer | 反向引物Reverse primer |
CGGC5_3865 | 193 | TTCTCCCTCCAGACCATCG | TGCAGACGTCCTGCTTCAA |
CGGC5_5363 | 185 | TGCCCGTGGATACTGAACAA | ATCCTTTGGCACTTCCTGCT |
CGGC5_7791 | 96 | CAAGGGCAACGGTCTCTTCT | CTGGGATCGTTGGCGTTTTC |
CGGC5_398 | 114 | CTCTACCCTCCTCACCGTCT | GAGTTGTTGCAGTGCTTCAG |
CGGC5_10060 | 122 | GCCGCCTCTTCCAATGCT | AAGTCCCCGTGTTGACTTC |
CGGC5_7871 | 65 | CACCTACTGCGACCCCTACTC | CCTTCTCAACACAGGCTCTGG |
CGGC5_462 | 409 | GCCTTTTTTGCCCTTTTGCTG | CAGGCTGTTCTTGTTGTCGGC |
CGGC5_15187 | 156 | TTTGACTGCAAGTGCAAGGACGAT | ACCACAGCAGGTCTTGAACGGGAA |
CGGC5_7737 | 64 | TCCGACTGCCACGACAACTA | TGCATCCAGAACGCTGAAGA |
Table 2
4 candidate effector gene-primer sequences designed for full-length cloning"
基因ID(编号) GenBank ID(number) | 扩增长度Amplification length/bp | 正向引物Forward primer | 反向引物Reverse primer |
CGGC5_3865(CfEP1) | 348 | ATGAAGTTCTCCCTCCAGACCAT | CTAGGCGCCAGGGGTGC |
CGGC5_7871(CfEP2) | 282 | ATGAAGGCTTTCACCGTCC | TTGGTGCACTTGCCATCC |
CGGC5_15187(CfEP3) | 243 | ATGCAGATTCAGAACGTTGTCC | TTATCGGCACAAGTCGAAGC |
CGGC5_7737(CfEP4) | 207 | ATGCTCTTCTCCAAGACCATCC | TTAGCAGTCGTCGCACATGA |
Table 3
31 Pathogenic related gene information"
基因ID GenBank ID | 基因名称Gene name | 突变类型Mutant phenotype | E-value | 分数Score | 功能Function |
CGGC5_14282 | SLP 1 | 效应物Effector | 1.17E-57 | 175 | 效应蛋白Effector protein |
CGGC5_14341 | SPM1 | 毒力降低Reduced virulence | 4.52E-64 | 210 | 蛋白酶活性Protease activity |
CGGC5_14887 | bcpme1 | 毒力降低Reduced virulence | 1.83E-74 | 240 | 果胶甲酯酶Pectin methylesterase |
CGGC5_15366 | SPM1 | 毒力降低Reduced virulence | 2.41E-69 | 225 | 蛋白酶活性Protease activity |
CGGC5_1639 | Ss-ggt1 | 毒力降低Reduced virulence | 1.94E-116 | 352 | γ-谷氨酰转肽酶Gamma-glutamyl transpeptidase |
CGGC5_1824 | PELA | 毒力降低Reduced virulence | 2.56E-62 | 196 | 果胶裂解酶Pectate lyase |
CGGC5_198 | MGG_10510 | 毒力降低Reduced virulence | 4.64E-116 | 335 | 假定蛋白Hypothetical protein |
CGGC5_243 | GAS1 | 毒力降低Reduced virulence | 6.19E-110 | 314 | 渗透Appressorial penetration |
CGGC5_2526 | SPM1 | 毒力降低Reduced virulence | 3.94E-60 | 202 | 蛋白酶活性Protease activity |
CGGC5_3265 | pnl1 | 毒力降低Reduced virulence | 5.10E-127 | 367 | 果胶裂解酶Pectin lyase |
CGGC5_3276 | CTB5 | 毒力降低Reduced virulence | 1.58E-43 | 157 | 氧化还原酶Oxidoreductase |
CGGC5_3706 | CPA1 | 毒力降低Reduced virulence | 3.01E-70 | 211 | 亲环蛋白Cyclophilin |
CGGC5_6227 | CMLE | 丧失致病性Loss of pathogenicity | 1.86E-62 | 201 | 羟基顺式、顺式粘康酸环化酶Carboxy-cis, cis-muconate cyclase |
CGGC5_6331 | Gas1 | 丧失致病性Loss of pathogenicity | 0 | 740 | 内置网葡萄糖苷酶Endoplasmic reticulum glucosidase |
CGGC5_6379 | IPP1 | 致死Lethal | 4.33E-71 | 224 | 无机焦磷酸酶Inorganic pyrophosphatase |
CGGC5_669 | PELA | 毒力降低Reduced virulence | 3.63E-63 | 196 | 果胶裂解酶Pectate lyase |
CGGC5_7177 | CTB5 | 毒力降低Reduced virulence | 7.97E-26 | 107 | 氧化还原酶Oxidoreductase |
CGGC5_7352 | CTB5 | 毒力降低Reduced virulence | 4.86E-48 | 169 | 氧化还原酶Oxidoreductase |
CGGC5_8160 | endo-1, 4-beta-xylanase | 毒力降低Reduced virulence | 0 | 527 | 内切-β-1,4-木聚糖酶Endo-β-1, 4-xylanase |
CGGC5_8220 | pnl1 | 毒力降低Reduced virulence | 1.13E-122 | 356 | 果胶裂解酶Pectin lyase |
CGGC5_9008 | lac2 | 毒力降低Reduced virulence | 2.23E-88 | 283 | 漆酶基因Laccase gene |
CGGC5_9093 | XYN11A | 毒力降低Reduced virulence | 6.19E-39 | 132 | 内切-β-1,4-木聚糖酶Endo-β-1, 4-xylanase |
CGGC5_9386 | CTB5 | 毒力降低Reduced virulence | 1.03E-26 | 110 | 氧化还原酶Oxidoreductase |
CGGC5_9512 | SPM1 | 毒力降低Reduced virulence | 1.89E-54 | 186 | 蛋白酶活性Protease activity |
CGGC5_11305 | MGG_04128 | 毒力降低Reduced virulence | 0 | 813 | 假定蛋白Hypothetical protein |
CGGC5_11629 | PELA | 毒力降低Reduced virulence | 8.23E-71 | 215 | 果胶裂解酶Pectate lyase |
CGGC5_13316 | FET3-1 | 毒力降低Reduced virulence | 2.10E-149 | 441 | 功能性铁氧化酶Functional ferroxidase |
CGGC5_13439 | PELD | 毒力降低Reduced virulence | 3.23E-92 | 268 | 果胶裂解酶Pectate lyase |
CGGC5_13646 | CYB2 | 毒力降低Reduced virulence | 4.52E-51 | 177 | 乳酸脱氢酶Lactate dehydrogenase |
CGGC5_13947 | Avenacinase gene | 丧失致病性Loss of pathogenicity | 0 | 840 | 阿唯那霉素酶Avenacinase |
CGGC5_14187 | Avenacinase gene | 丧失致病性Loss of pathogenicity | 6.17E-45 | 156 | 阿唯那霉素酶Avenacinase |
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