Table 1

Primers used in this study"

Fig.1

Domain prediction and phylogenetic analysis of CfAp1 A: The structure of CfAp1 was predicted using the SMART website (http://smart.embl-heidelberg.de/); BRLZ: basic-leucine zipper domain; the number represents the serial number of the amino acid. B: The phylogenetic tree was constructed using the neighbour-joining (NJ) method and analysed with 1 000 bootstrap replicates in Mega7.0; the number on the branch is the support calculated with 100 bootstrap replicates in the NJ analysis; the scale bar indicates the branch length. The number in front of each reference taxon is GenBank accession number of amino acid sequence orthologous to CfAp1. "

Fig.2

Generation of the gene deletion mutants of CfAP1 A: Schematic diagram of the deletion strategy for the CfAP1 gene. B: primer1 is bZIP45-5F/H855R, primer2 is bZIP45-7F/ bZIP45-8R; M: DL5000 marker; -: H2O negative control; +: WT positive control; ΔCfap1-8: mutant. "

Fig.3

Growth of CfAP1 gene-deletion mutants A: The wild-type strain (CFLH16), ΔCfap1-8, and complemented strains were inoculated on PDA media and cultured at 28 ℃ in the dark for 3 days. B: Colony side views are displayed. C: Statistical analysis of the colony diameter variations. Error bars are standard deviation and the same capitals represent no significant difference on P>0.05. "

Fig.4

The conidial quantitative statistical diagram of CfAP1 gene deleted mutants. Error bars are standard deviation and different lowercases represent significantly difference at P≤0.01. "

Fig.5

Oxygen stress sensitivity test of CfAP1 gene-deletion mutants A: The wild-type strain (CFLH16), ΔCfap1-8, and complemented strains were inoculated on PDA plates containing 0、2.5 and 5 mmol·L-1 H2O2, respectively. B: Statistical analysis of the inhibition rate of the indicated strains on different stressors. Error bars are standard deviation and different lowercases represent significantly difference at P≤0.01. "

Fig.6

Osmotic sensitivity test of CfAP1 gene-deletion mutants A: The wild-type strain (CFLH16), ΔCfap1-8, and complemented strains were inoculated on PDA plates containing 0.7 mol·L-1 NaCl. B: Statistical analysis of the inhibition rate of the indicated strains on different stressors. Error bars are standard deviation and different lowercases represent significantly difference at P < 0.01. "

Fig.7

Pathogenicity of CfAP1 gene deleted mutants to oil-tea leaves A and B: the wild type strain CFLH16, CfAP1 deletion mutant and the complemented strains were inoculated unwounded leaves and on wounded leaves; C: Statistical analysis of the disease lesion size of wounded leaves; D: Statistical analysis of the disease lesion size of unwounded leaves. Error bars are standard deviation and different lowercases represent significantly difference at P≤0.01. "

Fig.8

Appressorium in CfAP1 gene deleted mutants A:The CfAP1 gene deletion mutant can form a small amount appressorium; B: Statistical analysis of the rate of appressorium formation on glass surface; C:Appressorium collapse rate. Error bars are standard deviation and different capitals represent significantly difference at P≤0.05, different lowercases represent significantly difference at P≤0.01. "