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Scientia Silvae Sinicae ›› 2012, Vol. 48 ›› Issue (11): 49-56.doi: 10.11707/j.1001-7488.20121108

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Genetic Diversity in Natural Populations of Styrax tonkinensis

Li Nan1,2, Liu Xinhong2, Li Yingang2, Li Haibo2, Sheng Weitong1, Hui Gangying1, Zheng Yongqi1   

  1. 1. Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091;2. Zhejiang Academy of Forestry Hangzhou 310023
  • Received:2012-02-21 Revised:2012-06-10 Online:2012-11-25 Published:2012-11-25

Abstract:

Styrax tonkinensis is a woody species with multiple uses of timber, ornament, medicine and oil. We examined the genetic diversity and genetic structure of 20 populations which included 269 individuals of S.tonkinensis distributed in Yunnan, Guangdong, Guangxi, Jiangxi, Fujian, Hunan, and Guizhou Provinces of China, and Yen Bai of Vietnam by using SRAP markers. A total of 88 bands were amplified with 8 SRAP primers,of which 81 bands were polymorphic. Percentage of polymorphic bands (PPB) and Shannon’s phenotypic diversity index (Ho) were respectively 91.0% and 0.453 6 at the species level, 49.49% and 0.284 1 at the population level, reflecting a high level of genetic diversity. Analysis of molecular variance (AMOVA) revealed that the total genetic variation was 30.40% and 69.60% among populations and within populations, respectively. There was significant genetic differentiation within population and among populations. The Mantel test showed that the genetic distance was significantly positively correlated with the geographical distance.

Key words: Styrax tonkinensis, SRAP molecular marker, genetic differentiation, genetic diversity

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