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Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (12): 171-175.doi: 10.11707/j.1001-7488.20101228

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Genetic Diversity and Differentiation of Five Natural Populations of Artemisia halodendron

Wang Tiejuan, Li Weiqiong, Zhang Shuyan, Meng Xiangfei   

  1. College of Life Science and Technology, Inner Mongolia Normal University Hohhot 010022
  • Received:2009-10-26 Revised:2010-06-14 Online:2010-12-25 Published:2010-12-25

Abstract:

Artemisia halodendron is a dominant species of desert vegetation and an important sand-fixed plant in northeastern China. In this study, inter-simple sequence repeat (ISSR) markers were applied to investigate the genetic diversity and differentiation of five natural populations of A. halodendron . A total of 230 bands were amplified by 12 ISSR primers, of which 226 were polymorphic. Percentage of polymorphic bands (PPB) and Shannon's phenotypic diversity index ( Ho ) were respectively 98.26% and 0.329 6 at the species level,and 61.22% and 0.285 3 at the population level, showing a high level of genetic diversity. Analysis of molecular variance (AMOVA) revealed that 90.1% of the total genetic diversity occurred within populations. Based on the clustering analysis(UPGMA), the five populations were divided into two groups: the Hulunbuir Sandy Land group and the Horqin Sandy Land group. The Mantel test showed that genetic distance was significantly positively correlated with geographical distance.

Key words: Artemisia halodendron, ISSR, genetic diversity, genetic differentiation

CLC Number: