施氮对樟树林土壤微生物碳源代谢的影响

doi:10.11707/j.1001-7488.20140812

Abstract

Abstract:

Human activities are greatly changing the way and rate of nitrogen input to the land ecological system on a global scale,which has a significant impact on the structure and function of soil ecosystem. To clarify the carbon source utilization of soil microbial communities in response to nitrogen addition, a field experiment was conducted in a Cinnamomum camphora plantation in Hunan Forest Botanical Garden. Three levels of nitrogen addition were applied, such as control (0 g·m^-2), low nitrogen (5 g·m^-2) and high nitrogen (15 g·m^-2). Using the BIOLOG AN,FF,Gen Ⅲ and YT microplate methods, carbon source utilization of soil microbial communities (anaerobic bacteria, fungi, gram bacteria and yeast) was investigated after 1 year treatment under different nitrogen additions. The results showed:1)Metabolic activity of soil microbial communities with nitrogen additions reduced to different degrees,and the higher the nitrogen concentration,the weaker soil microbial metabolic activities.2)Diversity index analysis showed that nitrogen inputs affected the diversity of four microbial communites. The richness of the four soil microbial communities increased, but the evenness reduced, and the dominance of the anaerobic bacteria, fungi and gram bacteria reduced under nitrogen additions.3)The analysis of carbon source utilization showed that the utilization of other carbon sources had increased although the carbonhydrates were still the main carbon resources of microbe utilization after nitrogen inputs. Principal component analysis showed that different nitrogen additions had a great impact on the soil microbial communities. The carbon sources utilization of soil fungi and anaerobic bacteria was greatly influenced by nitrogen input, but the effects of gram bacteria and yeast were relatively weakly by the influence of nitrogen input.

Key words: soil microbial, nitrogen addition, carbon source utilization, diversity, Cinnamomum camphora plantation

CLC Number:

S714.3

Zhu Fan, Li Tianping, Yu Peiyi, Su Shaofeng, Hong Xiangqi, Chen Ting. Carbon Source Utilization of Soil Microbial Communities in Response to Nitrogen Addition in the Cinnamomum camphora Plantation[J]. Scientia Silvae Sinicae, 2014, 50(8): 82-89.

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Carbon Source Utilization of Soil Microbial Communities in Response to Nitrogen Addition in the Cinnamomum camphora Plantation

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