构树SRAP分子标记*

doi:10.11707/j.1001-7488.20091209

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (12): 54-58.doi: 10.11707/j.1001-7488.20091209

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SRAP Marker in Broussonetia papyrifera

Liu Zhiyuan^1,2,Fan Weihong^1,2,Shen Shihua¹

1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany，Chinese Academy of Sciences Beijing 100093； 2.Graduate University of Chinese Academy of Sciences Beijing 100039

Received:2008-11-12 Revised:1900-01-01 Online:2009-12-25 Published:2009-12-25
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Abstract

Abstract:

Broussonetia papyrifera is important with its environmental protection value and economic value. In this report，genetic diversity and genetic relationship were analyzed by SRAP marker. A DNA extraction protocol was developed for B. papyrifera and then an optimal SRAP-PCR system was established. The results showed that the bands ranged from 100 to 1 000 bp were clearly displayed. A total 439 bands were obtained by 17 pairs of primers, among which 319 bands were polymorphic, accounting for more than 72.6% of the total bands. The average Neis genetic diversity and Shannons information diversity index were respectively 0.227 5 and 0.133 6，which indicated genetic diversity of B. papyrifera was not high. The cluster analysis revealed there was relationship between the genetic variation and geographical distribution of B. papyrifera. Some specific bands were founded and could be converted into SCAR markers. This research would provide reference for making genetic map and breeding in B. papyrifera.

Key words: Broussonetia papyrifera, SRAP marker, genetic diversity, SCAR marker

CLC Number:

Liu Zhiyuan;Fan Weihong;Shen Shihua. SRAP Marker in Broussonetia papyrifera[J]. Scientia Silvae Sinicae, 2009, 12(12): 54-58.

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SRAP Marker in Broussonetia papyrifera

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