Abstract:

Phosphate-dissolving bacteria isolated from rhizosphere of mangrove were characterized by 16S rDNA PCR-RFLP and 16S rDNA sequence to describe the genetic diversity and phylogeny. The dendrogram of 16S rDNA PCR-RFLP revealed the 24 isolated stains were assembled at 59% similarity level. The strains were grouped into 7 subgroups at 80% similarity. Phylogenetic tree of 16S rDNA sequences was constructed with representative strains in reference to the homogenous sequences in Genebank, and the result showed that Bacillussp. was the dominant member,besides Paenibacillus sp.,Vibrio sp.，Klebsiella sp. and Pseudomonassp. were also found.The highly genetic diversity was highly revealed by this study.In addition, the phosphate-dissolving capacity to dissolve solubilize the unsolvable inorganic phosphorus of bacteria was tested.All representative strains had the phosphate-dissolving capacity.The pH of liquid media of strains both were reduced to some degree, but the correlations were not found between phosphate-dissolving capacity and pH of the culture solution.

Key words: mangrove, phosphate-dissolving bacteria, RFLP, phylogeny, diversity, phosphate-dissolving capacity