浙江马尾松毛虫不同地理种群间基因差异的ISSR分析

doi:10.11707/j.1001-7488.20080126

Scientia Silvae Sinicae ›› 2008, Vol. 44 ›› Issue (1): 164-168.doi: 10.11707/j.1001-7488.20080126

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Genetic Diversity among the Dendrolimus punctatus Populations Sampled From Different Region of Zhejiang Province

Weng Hongbiao¹,Niu Baolong¹,He Lihua¹,Jiang Ping²,Meng Zhiqi¹

1.Laboratory of Entomo-Molecular Biology,Zhejiang Academy of Agricultural Sciences Hangzhou 310021;2.Forest Pest Control and Quarantine Bureau of Zhejiang Province Hangzhou 310020

Received:2007-02-26 Revised:1900-01-01 Online:2008-01-25 Published:2008-01-25

Abstract

Abstract: Genetic diversity of Dendrolimus punctatus over five regions in Zhejiang Province were studied by ISSR-PCR.Thirteen primers were screened on the template of total DNA of the insects and total 74 bands of DNA fragments were amplified,of which the half(37 bands)was polymorphic.The genetic distance among the sampled populations ranged 0.114 4～0.475 4.The dendrogram was constructed based on their genetic distance using UPGMA method.The results indicated that there was low genetic diversity among the four populations from the main land,while the Dongtou population sampled from Dongtou island showed higher genetic diversity.

Key words: Dendrolimus punctatus, ISSR, genetic diversity, polymorphic

Weng Hongbiao;Niu Baolong;He Lihua;Jiang Ping;Meng Zhiqi. Genetic Diversity among the Dendrolimus punctatus Populations Sampled From Different Region of Zhejiang Province[J]. Scientia Silvae Sinicae, 2008, 44(1): 164-168.

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Genetic Diversity among the Dendrolimus punctatus Populations Sampled From Different Region of Zhejiang Province

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