Abstract:

Rhodiola sachalinensis calli were initiated from leaves on modified MS medium supplemented with 2 mg·L^-1 6-BA and 0.5 mg·L^-1 NAA. The calli were cooled at 4 ℃ for 8 h after treated with cryoprotectants (10%DMSO+0.5 mol·L^-1 sorbitol), and further refrigerated at a rate of 1 ℃·min^-1 to-40 ℃, and maintained at-40 ℃ for at least 2 h. The calli then were preserved in liquid nitrogen and thawed at 40 ℃ water bath. Calli were washed with liquid MS medium with 6-BA 2 mg·L^-1 and NAA 0.5 mg·L^-1 for several times, transferred to solid medium and cultured in darkness. Two weeks later, the calli were cultured in light (2 000 lx). The calli of R. sachalinensis showing vigorous growth were fresh and green after 5 weeks, and the survival rate reached 75.64%. Calli of R. sachalinensis could regenerate to plantlets.

Key words: Rhodiola sachalinensis, callus, plantlet regeneration