Abstract:

The callus of Tripterygium wilfordii root was used as materials, effects of different concentrations of 2，4-D，NAA, KT，or 6-BA and their combinations as well as carbon sources were investigated on callus growth and secondary metabolite contents of T. wilfordii. The results showed that 2，4-D promoted callus growth and accumulation of alkaloids. 6-BA inhibited callus growth, but obviously promoted triptolide accumulation. Although KT was suitable for callus growth，but inhibited significantly triptolide accumulation. The medium supplemented with 1.0 mg·L^-12，4-D and 0.5 mg·L^-1KT increased the callus growth, while addition of 1.0 mg·L^-12，4-D and 2.0 mg·L^-1KT increased the alkaloid content.The medium supplemented with 4.0 mg·L^-1 NAA and 0.5 mg·L^-16-BA increased the triptolide content. Sucrose was best for callus growth, maltose was best for triptolide accumulation, while glucose was best for total alkaloid accumulation.

Key words: Tripterygium wilfordii, callus, carbon sources, plant growth regulators, secondary metabolites