Abstract:

MS,B₅,NT,H,1/2MS,6,7-V and White were used as basic media,supplemented with 1.0 mg·L^-12,4-D and 0.5 mg·L^-1 KT, to study their effects on callus growth and secondary metabolite content of Tripterygium wilfordii.Light,pH,inoculating quantity,explants and culture time were studied with NT as basic media to explore the best culture conditions of the callus growth and secondary metabolite content.The results showed that 6,7-V medium was best for callus growth and preservation.White was the best suitable for the accumulation of triptolide and total alkaloids.NT medium was suitable for subculture.The optimal pH for the callus growth was 5.8.The optimal pH for the accumulation of triptolide was 6.7 and the optimal pH for the total alkaloids accumulation was 5.2.Dark condition was best not only for callus growth,but also for the triptolide and total alkaloids accumulation,with lesser browning callus.The root callus growth and triptolide content were significantly higher than that of the stem and leaf callus.Alkaloid content of leaf callus was significantly higher than that of the root and stem callus.The optimal duration for callus subculture was around 40-45 days.The triptolide and total alkaloids yield was the highest in 50 days.

Key words: Tripterygium wilfordii, callus, tissue culture, secondary metabolites