Abstract:

The organogenic calli of Slash Pine(Pinus elliottii) were used to study the differentiation, rooting and histological anatomy of adventitious buds.The result indicated that the best differentiation medium was TX+BA 0.5 mg·L^-1+IBA 0.5 mg·L^-1+30 g·L^-1 sucrose， and the best synchronous development medium was TX+IBA 0.5 mg·L^-1+30 g·L^-1 sucrose. The medium without major elements was benefit to rooting of adventitious buds in the beginning of root initiation, and the rooting cycle had been shorten and rooting rate had been improved when major elements were supplemented 25 days after root initiation. The organogenic calli had signs of white and semi-solid appearance outside and large number of meristem inside.

Key words: Slash Pine (Pinus elliottii), indirect organogenesis, plantlet regeneration, histological anatomy