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›› 2013, Vol. 49 ›› Issue (2): 33-38.doi: 10.11707/j.1001-7488.20130206

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Effects of Phytosulfokine on the Somatic Embryogenesis of Liriodendron hybrids(L. chinense×L. tulipifera)

Chen Jinhui, Zhang Yanjuan, Wu Yayun, Wang Pengkai, Wang Guangping, Shi Jisen   

  1. Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education College of Forest Resources and Environment, Nanjing Forestry University Nanjing 210037
  • Received:2012-07-25 Revised:2012-11-15 Online:2013-02-25 Published:2013-02-25

Abstract: Phytosulfokine (PSK),a new type of plant growth regulator, has multiple biological activities besides promoting single cell proliferation. In this paper, we imvestigated the role of PSK-α during somatic embryogenesis of Liriodendron hybrids(L. chinense×L. tulipifera) using immature zygotic embryos as explants. The result showed that PSK-α was able to improve cell division and proliferation and induce embryogenic cells formation. At the early stage of somatic embryogenesis, 0.1 mg·L-1 PSK-α increased the ratio of callus formation from explants and improved cell state; 0.5 mg·L-1 PSK-α induced embryogenic callus formation; and 0.8 mg·L-1 PSK-α was in favor of somatic embryo development and growth of regeneration plantlets.

Key words: Liriodendron hybrids, somatic embryogenesis, embryogenic callus, plantlet regeneration, PSK

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