翠菊‘花束绯红’下胚轴体胚发生及植株再生

doi:10.11707/j.1001-7488.20090829

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (8): 166-169.doi: 10.11707/j.1001-7488.20090829

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Somatic Embryogenesis and Plantlet Regeneration of Hypocotyl Explants of Callistephus chinensis ‘Bouquet Scarlet’

Du Li¹,Zhou Suo¹,Yang Jianwei¹,Yu Yali¹,Xie Yanhua¹,Bao Manzhu²

1.School of Life Science and Technology， Nanyang Normal UniversityNanyang 473000; 2.Key Laboratory of Horticultural Plant Biology of Ministry of EducationCollege of Horticulture and Forestry Sciences， Huazhong Agricultural UniversityWuhan 430070

Received:2008-07-24 Revised:1900-01-01 Online:2009-08-25 Published:2009-08-25
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Abstract

Abstract:

This paper reported the successful embryogenic callus induction and somatic embryo plantlet regeneration from hypocotyls of Callistephus chinensis ‘Bouquet Scarlet’. Experimental results indicated that plant hormone types and their concentrations， carbohydrate sources and sucrose concentrations had varied effect on embryogenic callus induction from hypocotyls. The appropriate medium for embryogenic callus induction of C. chinensis ‘Bouquet Scarlet’ was MS+ 0.5 mg·L^-16-BA + 0.5 mg·L^-1 IBA， and with the medium the embryogenic callus induction rate was 27.1％.Sucrose at 30 g·L^-1 was the suitable carbohydrate source and concentration. The suitable medium for plantlet regeneration of C. chinensis ‘Bouquet Scarlet’ embryogenic calli was MS+0.5 mg·L^-1 6-BA + 0.5 mg·L^-1 IBA + 550 mg·L^-1 proline， and with the medium the plantlet regeneration rate was 46.7%.

Key words: Callistephus chinensis &lsquo, Bouquet Scarlet&rsquo, hypocotyl, embryogenic callus, plantlet regeneration

CLC Number:

Du Li;Zhou Suo;Yang Jianwei;Yu Yali;Xie Yanhua;Bao Manzhu. Somatic Embryogenesis and Plantlet Regeneration of Hypocotyl Explants of Callistephus chinensis ‘Bouquet Scarlet’[J]. Scientia Silvae Sinicae, 2009, 12(8): 166-169.

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Somatic Embryogenesis and Plantlet Regeneration of Hypocotyl Explants of Callistephus chinensis ‘Bouquet Scarlet’

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